Ambient temperature influences aging in an annual fish (Nothobranchius rachovii)
Ambient temperature influences aging in an annual fish (Nothobranchius rachovii)
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DOI：
10.1111/j.1474-9726.2009.00525.x
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发表时间：
2009-12-01
期刊：
影响因子：
7.8
通讯作者：
Chiu, Ya-Chi
中科院分区：
文献类型：
--
作者：
Hsu, Chin-Yuan;Chiu, Ya-Chi
通过降低栖息地的环境温度来延长寿命已经在多种生物中得到证实。然而，其机制仍然难以捉摸。在这项研究中，我们研究了一年生鱼（Nothobranchius rachovii）在高（30摄氏度），中（25摄氏度）和低（20摄氏度）的环境温度下饲养的存活率和老化过程。结果表明，低环境温度延长存活率，而高环境温度缩短存活率。在低环境温度下，表达衰老相关的β-半乳糖苷酶，脂褐素，活性氧，脂质过氧化，蛋白质氧化，线粒体密度和ADP/ATP比相比，在高和中等温度下饲养，而过氧化氢酶活性，锰超氧化物歧化酶活性，线粒体膜电位和水平的ATP，ADP，Sirt 1和叉头盒O的表达升高。Hsp 70和CIRP的表达水平在所测试的任何环境温度下均未显示出显著差异。我们的结论是，细胞代谢，能量利用和基因表达的改变，在较低的环境温度下，这是与一年生鱼类的寿命延长。
P>Extending lifespan by lowering ambient temperature in the habitat has been shown in a variety of organisms. Its mechanism, however, remains elusive. In this study, we examined the survivorship and the aging process of the annual fish (Nothobranchius rachovii) reared under high (30 degrees C), moderate (25 degrees C) and low (20 degrees C) ambient temperatures. The results showed that low ambient temperatures prolong survivorship, whereas high ambient temperatures shorten survivorship. At low ambient temperature, expression of senescence-associated beta-galactosidase, lipofuscin, reactive oxygen species, lipid peroxidation, protein oxidation, mitochondrial density and ADP/ATP ratio were reduced compared with those reared at high and moderate temperatures, whereas catalase activity, Mn-superoxide dismutase activities, mitochondrial membrane potential and the levels of ATP, ADP, Sirt1 and Forkhead box O expression were elevated. The expression levels of Hsp70 and CIRP showed no significant difference under any of the ambient temperatures tested. We concluded that cellular metabolism, energy utilization and gene expression are altered at lower ambient temperature, which is associated with the extension of lifespan of the annual fish.