Encapsulation of Plant Growth-Promoting Bacterial Crude Extract in Nanoliposome and Its Antifungal Property Against Fusarium oxysporum
Encapsulation of Plant Growth-Promoting Bacterial Crude Extract in Nanoliposome and Its Antifungal Property Against Fusarium oxysporum
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DOI：
10.1021/acsagscitech.1c00188
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发表时间：
2021-12-20
期刊：
影响因子：
--
通讯作者：
Kohli, Punit
中科院分区：
文献类型：
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作者：
Atienza, Ma Theresa Jonna A.;Magpantay, Ma Danica A.;Kohli, Punit
纳米脂质体包裹的新型生物杀菌剂的合成、表征及其对镰刀菌的抑菌活性。描述了将植物生长促进菌（PGPB）乙醇粗提物包裹于纳米聚合脂质体中，制备了纳米杀菌剂，并通过紫外可见光谱、原子力显微镜（AFM）和透射电子显微镜（TEM）对纳米杀菌剂进行了表征。TEM和UV-vis测量证实了纳米脂质体中生物杀真菌剂的包封。尽管发现纳米脂质体和PGPB粗提取物对研究中研究的三种真菌无效，但体外生长抑制测定显示纳米生物杀真菌剂可抑制66 +/-3%尖孢镰刀菌fsp的菌丝体生长。古巴热带种族1（Foc TR 1）.它还有效地抑制44 +/- 4%的尖孢镰刀菌fsp。黄瓜属（Cucumerinum）。纳米生物杀真菌剂对番茄枯萎病菌（Fusarium oxysporum f.sp. lycopersici，Fol）菌丝生长的抑制作用是适度的（19 +/-2%）。在初步结果的基础上，纳米生物杀菌剂对Foc TR 1和Focu表现出有效的抑制作用。纳米生物杀菌剂的优化是一个潜在的替代生物控制选项，用于控制镰刀菌。纳米生物杀菌剂对真菌的抑制机制的可能模式进行了讨论。
Synthesis and characterization of a novel biofungicide encapsulated in nanoscale liposome particles (referred as nanobiofungicide) and its antifungal activity against Fusarium spp. are described. The nanobiofungicide was synthesized by encapsulating ethanol crude extract from plant growth-promoting bacteria (PGPB) into nanoscale polymerized liposomes, and the nanobiofungicides were characterized through UV-vis spectroscopy, atomic force microscopy (AFM), and transmission electron microscopy (TEM). TEM and UV-vis measurements confirmed encapsulation of biofungicides in nanoliposomes. Whereas nanoliposomes and PGPB crude extract were found to be ineffective against three fungi investigated in studies, in vitro growth inhibition assays revealed that the nanobiofungicide can inhibit 66 +/- 3% mycelial growth of Fusarium oxysporum fsp. cubense Tropical Race 1 (Foc TR1). It also effectively inhibited 44 +/- 4% of Fusarium oxysporum fsp. cucumerinum (Focu). Inhibitory effects of nanobiofungicide were modest (19 +/- 2%) against Fusarium oxysporum f.sp. lycopersici (Fol) mycelial growth. On the basis of preliminary results, the nanobiofungicide showed an effective inhibitory effect against Foc TR1 and Focu. The optimization of nanobiofungicide is potentially a viable alternative biocontrol option for controlling Fusarium spp. Possible modes of an inhibition mechanism of nanobiofungicides against fungi are also discussed.