A new mechanism for reduced cell adhesion: Adsorption dynamics of collagen on a nanoporous gold surface
A new mechanism for reduced cell adhesion: Adsorption dynamics of collagen on a nanoporous gold surface
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DOI：
10.1016/j.msec.2020.111461
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发表时间：
2021-02-01
影响因子：
7.9
通讯作者：
Mabuchi, Mamoru
中科院分区：
文献类型：
--
作者：
Deguchi, Soichiro;Yokoyama, Ryo;Mabuchi, Mamoru
纳米结构材料如纳米颗粒和纳米多孔材料强烈影响细胞行为如细胞活力。由于细胞摄取纳米多孔材料不会发生，纳米多孔材料对细胞的影响机制与纳米颗粒不同。纳米多孔材料对细胞的影响被认为是由纳米多孔材料诱导的细胞外基质（ECM）中的大的构象变化引起的，尽管机械转导和临界粘着斑簇大小也对细胞反应有影响。然而，我们表明，间充质干细胞的粘附到金表面的纳米多孔金（NPG）减少，尽管由NPG诱导的胶原蛋白的构象变化低于实验分析的检测限。通过分子动力学模拟研究胶原蛋白在NPG上的吸附动力学，以确定细胞粘附NPG减少的起源。胶原蛋白在NPG上的吸附能低于在平面金（FG）上的吸附能，尽管NPG和FG上吸附的胶原蛋白片段的整体构象之间的差异不大。这一发现与NPG的表面应变和胶原蛋白氨基酸由于链间氢键的有限运动有关。在这项研究中获得的结果提供了新的见解纳米结构材料和ECM之间的相互作用。
Nanostructured materials such as nanoparticles and nanoporous materials strongly affect cell behaviors such as cell viability. Because cellular uptake of nanoporous materials does not occur, mechanisms for the effects of nanoporous materials on cells are different from those of nanoparticles. The effects of nanoporous materials on cells are thought to result from large conformational changes in the extracellular matrix (ECM) induced by the nanoporous materials, although the mechanotransduction and the critical focal adhesion cluster size also have an effect on the cell response. However, we show that the adhesion of mesenchymal stem cells to a gold surface is reduced for nanoporous gold (NPG), despite the conformational changes in collagen induced by NPG being below the detection limits of the experimental analyses. The adsorption dynamics of collagen on NPG are investigated by molecular dynamics simulations to determine the origin of the reduced cell adhesion to NPG. The adsorption energy of collagen on NPG is lower than that on flat gold (FG) despite there being little difference between the global conformation of collagen segments adsorbed on NPG compared with FG. This finding is related to the surface strain of NPG and the limited movement of collagen amino acids owing to interchain hydrogen bonds. The results obtained in this study provide new insight into the interactions between nanostructured materials and the ECM.