Roles of EphrinB2 and EphB4 in Alveolar Bone under Initial Compressive Mechanical Stress of Dental Implant Replacement
Roles of EphrinB2 and EphB4 in Alveolar Bone under Initial Compressive Mechanical Stress of Dental Implant Replacement
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发表时间：
2018
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通讯作者：
Replacement
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Replacement
最近发现ephrin/Eph家族之间的交流参与了机械应力下的细胞分化。为了了解EphrinB2和EphB4在牙种植体置换术初始机械应力下成骨细胞和牙槽骨中的作用，我们首先在体外对MC3T3-E1细胞进行周期性压缩或广泛受力。采用实时RT-PCR或western blotting检测EphrinB2和EphB4的表达，同时检测Osterix作为成骨细胞分化标志物的表达。接下来，采用实时RT-PCR检测EphrinB2或EphB4固定的微型种植体表面对种植体周围牙槽骨编码Osterix基因表达的影响。压缩条件下培养的MC3T3-E1细胞Osterix mRNA表达较正常条件下降低。在压缩条件下培养的细胞中，EphrinB2和EphB4 mRNA和蛋白水平均升高。最初，与对照组相比，扭矩机械应力降低了微型种植体周围牙槽骨中Osterix mRNA的表达。与对照组相比，10 Nmm扭矩使EphB4 mRNA表达增加，10和20 Nmm扭矩使EphrinB2 mRNA表达增加。用EphrinB2-Fc固定的微型种植体表面恢复了Osterix的抑制。综上所述，本研究结果表明，在初始机械力（尤其是压缩力）作用下，成骨细胞中EphrinB2和EphB4的结合增加可能参与了牙槽骨的恢复，并可能导致替代种植体的初级固定逐渐减少，最终导致稳定。
The communication between ephrin/Eph families was recently shown to be involved in cell differentiation under mechanical stress. To understand the roles of EphrinB2 and EphB4 in osteoblast cells and alveolar bone under initial mechanical stress of dental implant replacement, first, MC3T3-E1 cells were subjected to cyclical compressive or extensive force in vitro . The expressions of EphrinB2 and EphB4 were examined by real-time RT-PCR or western blotting, together with expression of Osterix as an osteoblast differentiation marker. Next, the effects of mini-implant surfaces immobilised with EphrinB2 or EphB4 on the expression of the gene encoding Osterix in alveolar bone surrounding implants were examined by real-time RT-PCR. Osterix mRNA expression was decreased in MC3T3-E1 cells cultured under compressive conditions, compared with that under normal conditions. The mRNA and protein levels of EphrinB2 and EphB4 were increased in the cells cultured under compressive conditions. Initially, mechanical stress by torques decreased Osterix mRNA expression in alveolar bone surrounding mini-implants, compared with the control groups. Torque of 10 Nmm increased EphB4 mRNA expression compared with the control groups, while torques of 10 and 20 Nmm increased EphrinB2 mRNA expression. The mini-implant surface immobilised with EphrinB2-Fc recovered the suppression of Osterix. Taken together, the present results suggest that increased binding of EphrinB2 and EphB4 in osteoblast cells exposed to initial mechanical force, especially compressive force, may be involved in alveolar bone recovery, and may cause a gradual decrease of the primary fixation in a replaced dental implant and finally lead to stability.