Phosphorylation of gamma-aminobutyrate (GABA)/benzodiazepine receptors by cyclic AMP-dependent protein kinase.
Phosphorylation of gamma-aminobutyrate (GABA)/benzodiazepine receptors by cyclic AMP-dependent protein kinase.
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环 AMP 依赖性蛋白激酶对 γ-氨基丁酸 (GABA)/苯二氮卓受体进行磷酸化。
DOI：
10.1042/bj2590613
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发表时间：
1989
期刊：
影响因子：
--
通讯作者：
Turner,AJ
中科院分区：
文献类型：
--
作者：
Kirkness,EF;Bovenkerk,CF;Ueda,T;Turner,AJ
猪大脑皮层γ -氨基丁酸(GABA)/苯二氮卓受体的制备由三个主要的多肽带（51、55和57 kDa）组成，其纯化比例约为。分别为2:1:1。用环amp依赖性蛋白激酶处理纯化的受体制剂，导致32P仅在55kda带内主要掺入。最大掺入量为0.6 mol 32P/mol 55 kDa多肽。磷酸化的受体亚基（β亚基）与GABA受体激动剂[3H]muscimol不可逆标记的带显示相同的明显Mr。这两个非磷酸化的亚基多肽（51和57 kDa）分别被[3H]氟硝西泮不可逆地标记，并被α亚基特异性的抗肽抗体识别。
Preparations of gamma-aminobutyrate (GABA)/benzodiazepine receptor from pig cerebral cortex are composed of three major bands of polypeptides (51, 55 and 57 kDa) which are purified in a ratio of approx. 2:1:1 respectively. Treatment of purified receptor preparations with cyclic AMP-dependent protein kinase resulted in major incorporation of 32P into the 55 kDa band only. The maximum incorporation achieved was 0.6 mol of 32P/mol of 55 kDa polypeptide. The phosphorylated receptor subunit (beta-subunit) displays the same apparent Mr as a band labelled irreversibly with the GABA receptor agonist [3H]muscimol. The two nonphosphorylated subunit polypeptides (51 and 57 kDa) are each labelled irreversibly with [3H]flunitrazepam and are recognized by anti-peptide antibodies specific for alpha-subunits.