Use of the Chinchilla Model for Nasopharyngeal Colonization To Study Gene Expression by Moraxella catarrhalis
Use of the Chinchilla Model for Nasopharyngeal Colonization To Study Gene Expression by Moraxella catarrhalis
复制标题
使用龙猫鼻咽定植模型研究卡他莫拉氏菌的基因表达
作者：
T. Hoopman;Wei Liu;Stephanie N. Joslin;Christine Pybus;Jennifer L Sedillo;Maria Labandeira;C. Laurence;Wei Wang;J. Richardson;L. Bakaletz;E. Hansen
通过鼻腔途径无创接种卡他莫拉菌。从这些M.卡他感染的动物显示在上皮内存在显著的炎症。在假接种动物中没有类似的组织病理学发现，证实了M。在这种环境中，卡他暴露于显著的宿主衍生因子。接种后24小时，活M.从动物的鼻腔和鼻咽中回收数量足以用于DNA微阵列分析的卡他微生物。超过100米。卡他病基因在体内上调，包括编码参与截短的脱氮途径或氧化应激反应的蛋白质的开放阅读框（ORF），以及几种推定的转录调节因子。另外，200米。当将该细菌引入鼻咽时，发现卡他基因下调。这些下调的基因包括编码几个特征性M.卡他炎表面蛋白包括Hag、McaP和MchA 1。实时逆转录酶PCR（RT-PCR）被用作严格的控制，以验证体内基因表达模式的结果，通过DNA微阵列分析测量。其中一个在体内上调的基因（MC ORF 1550）的失活导致M.卡他菌在灰鼠鼻咽中存活超过3天。这是M.卡他细胞。
Young adult chinchillas were atraumatically inoculated with Moraxella catarrhalis via the nasal route. Detailed histopathologic examination of nasopharyngeal tissues isolated from these M. catarrhalis-infected animals revealed the presence of significant inflammation within the epithelium. Absence of similar histopathologic findings in sham-inoculated animals confirmed that M. catarrhalis was exposed to significant host-derived factors in this environment. Twenty-four hours after inoculation, viable M. catarrhalis organisms were recovered from the nasal cavity and nasopharynx of the animals in numbers sufficient for DNA microarray analysis. More than 100 M. catarrhalis genes were upregulated in vivo, including open reading frames (ORFs) encoding proteins that are involved in a truncated denitrification pathway or in the oxidative stress response, as well as several putative transcriptional regulators. Additionally, 200 M. catarrhalis genes were found to be downregulated when this bacterium was introduced into the nasopharynx. These downregulated genes included ORFs encoding several well-characterized M. catarrhalis surface proteins including Hag, McaP, and MchA1. Real-time reverse transcriptase PCR (RT-PCR) was utilized as a stringent control to validate the results of in vivo gene expression patterns as measured by DNA microarray analysis. Inactivation of one of the genes (MC ORF 1550) that was upregulated in vivo resulted in a decrease in the ability of M. catarrhalis to survive in the chinchilla nasopharynx over a 3-day period. This is the first evaluation of global transcriptome expression by M. catarrhalis cells in vivo.
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DOI：
10.1164/rccm.200412-1747oc
发表时间：
2005-07-15
影响因子：
24.7
作者：
Murphy, TF;Brauer, AL;Sethi, S
通讯作者：
Sethi, S
影响因子：
3.5
作者：
HORTON, RM;HUNT, HD;PEASE, LR
通讯作者：
PEASE, LR
影响因子：
120.7
作者：
Hall-Stoodley, Luanne;Hu, Fen Ze;Kerschner, Joseph E.
通讯作者：
Kerschner, Joseph E.
DOI：
10.1073/pnas.1106341108
发表时间：
2011-09-13
影响因子：
11.1
作者：
Agnew, Christopher;Borodina, Elena;Brady, R. Leo
通讯作者：
Brady, R. Leo