CLONING THE CHROMOSOMAL BREAKPOINT OF T(14-18) HUMAN LYMPHOMAS - CLUSTERING AROUND JH ON CHROMOSOME-14 AND NEAR A TRANSCRIPTIONAL UNIT ON 18
CLONING THE CHROMOSOMAL BREAKPOINT OF T(14-18) HUMAN LYMPHOMAS - CLUSTERING AROUND JH ON CHROMOSOME-14 AND NEAR A TRANSCRIPTIONAL UNIT ON 18
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DOI：
10.1016/s0092-8674(85)80070-2
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发表时间：
1985-01-01
期刊：
影响因子：
64.5
通讯作者：
KORSMEYER, SJ
中科院分区：
文献类型：
--
作者：
BAKHSHI, A;JENSEN, JP;KORSMEYER, SJ
在不同的肿瘤中发现的特定染色体易位表明，这些断裂点侧翼的基因在转化中起着关键作用。超过60%的人类滤泡性淋巴瘤存在t（14;18）（q32;q21）染色体易位。一个意想不到的重排的IG H链基因被expoloied克隆染色体断裂点。从18 q21中分离的一种元件在所有4个t（14;18）携带细胞系和11个滤泡性淋巴瘤中的6个中介导易位，但在其他B或非B细胞中通常不重排。断裂点聚集在18号染色体上的一个小的4.3 kb区域内。14号染色体上的断裂点集中在JH的5“以内或紧邻JH。这些断裂点保留了IG增强子区域，该区域靠近染色体片段18 q21上鉴定的新转录单位。由于没有已知的细胞癌基因映射到18 q21，克隆这个元素提供了一个机会，以表征一个潜在的新的转化基因。
Specific chromosomal translocations found in distinct neoplasms suggest that genes that flank such breakpoints play a critical role in transformation. The t(14;18)(q32;q21) chromosomal translocation present in over 60% of human follicular lymphomas was characterized. An unexpected rearrangement of an Ig H-chain gene was expoloited to clone the chromosomal breakpoint. An element isolated from 18q21 mediated translocations in all 4 t(14;18) bearing cell lines and in 6 of 11 follicular lymphomas, but did not normally rearrange in other B or non-B cells. The breakpoints clustered within a small 4.3 kb [kilobase] region on chromosome 18. The breakpoints on chromosome 14 were focused within or immediately 5'' to JH. These breakpoints retained the Ig enhancer region close to a new transcriptional unit identified on chromosome segment 18q21. Since none of the cellular oncogenes are known to map to 18q21, cloning this element provides an opportunity to characterize a potentially new transforming gene.