A gentle mechanical skin stimulation technique for inhibition of micturition contractions of the urinary bladder
A gentle mechanical skin stimulation technique for inhibition of micturition contractions of the urinary bladder
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DOI：
10.1016/j.autneu.2011.11.002
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发表时间：
2012-04-03
影响因子：
2.7
通讯作者：
Kasuya, Yutaka
中科院分区：
文献类型：
--
作者：
Hotta, Harumi;Masunaga, Koichi;Kasuya, Yutaka
在麻醉的雄性大鼠上，观察了不同节段性皮肤刺激对膀胱排尿收缩的影响。通过尿道插管注入生理盐水使膀胱扩张，直到膀胱产生节律性排尿收缩，这是膀胱盆腔传出神经节律性爆发放电的结果。通过用弹性体“辊”在皮肤顶部缓慢滚动来施加温和刺激1分钟。在会阴区滚动抑制排尿收缩和盆腔传出放电期间和刺激后。刺激后肢，腹部和前肢抑制排尿收缩刺激结束后，在这个顺序的有效性。在刺激会阴部皮肤时，膀胱扩张至恒定压力时盆腔传出放电的反射性增加也被抑制至其对照反应的45%。会阴刺激引起的排尿收缩的抑制被取消，在很大程度上由阿片受体拮抗剂纳洛酮和完全切断支配会阴皮肤的皮神经。我们记录了阴部神经皮支的单一传入活动，发现刺激激发的纤维是低阈值机械感受性A β，A δ和C纤维。C纤维（7.9Hz）的放电频率显著高于A β（2.2Hz）和A δ（2.9Hz）的放电频率。结果表明，低阈值皮肤机械感受性有髓和无髓纤维的低频激发抑制膀胱盆腔副交感神经反射，主要是通过释放阿片类药物，导致抑制排尿收缩。(C)2011 Elsevier B.V.保留所有权利。
Effects of gentle skin stimulation of various segmental areas on the micturition contractions of the urinary bladder were examined in anesthetized male rats. The bladder was expanded by infusing saline via urethral cannula until the bladder produced rhythmic micturition contractions as a consequence of rhythmic burst discharges of vesical pelvic efferent nerves. Gentle stimulation was applied for 1 min by slowly rolling on top of skin with an elastomer "roller". Rolling on the perineal area inhibited both micturition contractions and pelvic efferent discharges during and after stimulation. Stimulation of the hindlimb, abdomen and forelimb inhibited micturition contractions after stimulation ended, in this order of effectiveness. During stimulation of the perineal skin, the reflex increase in pelvic efferent discharges in response to bladder distension to a constant pressure was also inhibited up to 45% of its control response. The inhibition of the micturition contractions induced by perineal stimulation was abolished, to a large extent by the opioid receptor antagonist naloxone and completely by severing cutaneous nerves innervating the perineal skin. We recorded unitary afferent activity from cutaneous branches of the pudendal nerve and found that the fibers excited by stimulation were low-threshold mechanoreceptive A beta, A delta and C fibers. Discharge rates of afferent C fibers (7.9 Hz) were significantly higher than those of A beta (2.2 Hz) and A delta (2.9 Hz) afferents. The results suggest that low frequency excitation of low threshold cutaneous mechanoreceptive myelinated and unmyelinated fibers inhibits a vesico-pelvic parasympathetic reflex, mainly via release of opioids, leading to inhibition of micturition contraction. (C) 2011 Elsevier B.V. All rights reserved.