Oesophageal epithelial innervation in health and reflux oesophagitis
Oesophageal epithelial innervation in health and reflux oesophagitis
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DOI：
10.1136/gut.44.3.317
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发表时间：
1999-03-01
期刊：
影响因子：
24.5
通讯作者：
Burnstock, G
中科院分区：
文献类型：
--
作者：
Newton, M;Kamm, MA;Burnstock, G
背景-食管对胃内容物反流的反应可能依赖于食管粘膜中完整的神经机制。上皮神经支配尚未进行系统的评估，在健康或反流diseases. Aims-To研究食管上皮神经支配的控制，也发炎和非发炎粘膜的患者反流性食管炎和愈合oesophagitis. Patients-10对照，9例反流性食管炎，和5例愈合oesophagitis. Methods-食管上皮活检标本在内镜下获得。用免疫组化方法观察神经元标记蛋白基因产物9.5（PGP）、降钙素基因相关肽（CGRP）、神经肽Y（NPY）、P物质（SP）和血管活性肠肽（VIP）在脑内的分布。神经支配的密度进行了评估，在每个食管上皮活检标本中含有免疫反应性纤维（发现在subepithelium和上皮乳头，但不穿透epithelium）的乳头的比例PGP免疫反应性神经纤维阳性的乳头的比例显着增加，与对照组相比，非发炎和愈合的组织。上皮乳头内VIP免疫反应纤维也显著增加。其他neuropeptides显示没有成比例的变化inflammation. Conclusions-上皮活检标本可用于评估神经支配的粘膜的神经支配是不改变的非炎症组织的食管炎患者，但改变炎症反应，有一个选择性增加（约三至四倍）VIP含食管。食管神经
Background-The response of the oesophagus to refluxed gastric contents is likely to depend on intact neural mechanisms in the oesophageal mucosa. The epithelial innervation has not been systematically evaluated in health or reflux disease.Aims-To study oesophageal epithelial innervation in controls, and also inflamed and non-inflamed mucosa in patients with reflux oesophagitis and healed oesophagitis.Patients-Ten controls, nine patients with reflux oesophagitis, and five patients with healed oesophagitis.Methods-Oesophageal epithelial biopsy specimens were obtained at endoscopy. The distribution of the neuronal marker protein gene product 9.5 (PGP), and the neuropeptides calcitonin gene related peptide (CGRP), neuropeptide Y (NPY), substance P (SP), and vasoactive intestinal peptide (VIP) were investigated by immunohistochemistry. Density of innervation was assessed by the proportion of papillae in each oesophageal epithelial biopsy specimen containing immunoreactive fibres (found in the subepithelium and epithelial papillae, but not penetrating the epithelium).Results-The proportion of papillae positive for PGP immunoreactive nerve fibres was significantly increased in inflamed tissue when compared with controls, and non-inflamed and healed tissue. There was also a significant increase in VIP immunoreactive fibres within epithelial papillae. Other neuropeptides showed no proportional changes in inflammation.Conclusions-Epithelial biopsy specimens can be used to assess innervation in the The innervation of the mucosa is not altered in non-inflamed tissue of patients with oesophagitis but alters in response to inflammation, where there is a selective increase (about three- to fourfold) in VIP containing oesophagus. oesophageal nerves.