Titanium dioxide nanoparticles oral exposure to pregnant rats and its distribution
Titanium dioxide nanoparticles oral exposure to pregnant rats and its distribution
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DOI：
10.1186/s12989-019-0313-5
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发表时间：
2019-07-18
影响因子：
10
通讯作者：
Yu, Wook-Joon
中科院分区：
文献类型：
--
作者：
Lee, Jinsoo;Jeong, Ji-Seong;Yu, Wook-Joon
背景资料：二氧化钛（TiO2）纳米颗粒是工业中最人工制造的纳米材料之一，用于食品，牙膏，化妆品和油漆。孕妇及其孕体可能会接触到二氧化钛纳米颗粒;然而，这些纳米颗粒在怀孕期间的潜在影响是有争议的，其内部分布尚未研究。因此，在这项研究中，我们研究了口服暴露于TiO2纳米颗粒的潜在影响及其在妊娠期间的分布。将TiO2纳米颗粒经口给予妊娠Sprague-Dawley大鼠（每组12只雌性动物），从妊娠第6天（GD）至第19天，以0、100、300和1000 mg/kg/天剂量水平给药，然后在GD 20进行剖宫产。结果：在母体和胚胎-胎仔检查中，在一般临床体征、体重、摄食量、器官重量、肉眼检查结果剖宫产参数和胎儿形态学检查。结论：妊娠期口服TiO2纳米颗粒可增加母体肝脏、脑和胎盘中钛的含量，但不会对母体产生明显的毒性作用，也不会影响胚胎-胎仔的发育。这些结果可用于评估妊娠期间口服TiO2纳米颗粒的人体风险评估，考虑到复杂暴露场景的可能性和TiO2纳米颗粒的各种尺寸，认为有必要进行额外的全面毒性研究。
Background: Titanium dioxide (TiO2) nanoparticles are among the most manufactured nanomaterials in the industry, and are used in food products, toothpastes, cosmetics and paints. Pregnant women as well as their conceptuses may be exposed to TiO2 nanoparticles; however, the potential effects of these nanoparticles during pregnancy are controversial, and their internal distribution has not been investigated. Therefore, in this study, we investigated the potential effects of oral exposure to TiO2 nanoparticles and their distribution during pregnancy. TiO2 nanoparticles were orally administered to pregnant Sprague-Dawley rats (12 females per group) from gestation days (GDs) 6 to 19 at dosage levels of 0, 100, 300 and 1000 mg/kg/day, and then cesarean sections were conducted on GD 20.Results: In the maternal and embryo-fetal examinations, there were no marked toxicities in terms of general clinical signs, body weight, food consumption, organ weights, macroscopic findings, cesarean section parameters and fetal morphological examinations. In the distribution analysis, titanium contents were increased in the maternal liver, maternal brain and placenta after exposure to high doses of TiO2 nanoparticles.Conclusion: Oral exposure to TiO2 during pregnancy increased the titanium concentrations in the maternal liver, maternal brain and placenta, but these levels did not induce marked toxicities in maternal animals or affect embryo-fetal development. These results could be used to evaluate the human risk assessment of TiO2 nanoparticle oral exposure during pregnancy, and additional comprehensive toxicity studies are deemed necessary considering the possibility of complex exposure scenarios and the various sizes of TiO2 nanoparticles.