Intermodular communication in polyketide synthases: comparing the role of protein-protein interactions to those in other multidomain proteins.
Intermodular communication in polyketide synthases: comparing the role of protein-protein interactions to those in other multidomain proteins.
复制标题
聚酮合酶中的模块间通讯：将蛋白质-蛋白质相互作用的作用与其他多结构域蛋白质中的作用进行比较。
DOI：
10.1021/bi002462v
复制
发表时间：
2001
期刊：
影响因子：
2.9
通讯作者：
Khosla,C
中科院分区：
文献类型：
--
作者：
Tsuji,SY;Wu,N;Khosla,C
尽管蛋白质-蛋白质相互作用在生物系统内转导信号中的作用已被广泛探索，但它们与代谢中间产物通道的相关性尚未得到广泛认识。聚酮合成酶（pks）和非核糖体肽合成酶（NRPSs）是两个相关的模块化大合成酶家族，它们将共价结合的中间体从一个活性位点引导到另一个活性位点。最近的生物化学研究强调了蛋白质-蛋白质相互作用在这些链传递过程中的重要性。本文回顾了关于这一主题的现有信息，并将其可能的机制含义与选定的经过充分研究的多组分蛋白质系统进行了比较。
Although the role of protein−protein interactions in transducing signals within biological systems has been extensively explored, their relevance to the channeling of intermediates in metabolism is not widely appreciated. Polyketide synthases (PKSs) and nonribosomal peptide synthetases (NRPSs) are two related families of modular megasynthases that channel covalently bound intermediates from one active site to the next. Recent biochemical studies have highlighted the importance of protein−protein interactions in these chain transfer processes. The information available on this subject is reviewed, and its possible mechanistic implications are placed in context by comparisons with selected well-studied multicomponent protein systems.