Using highly recyclable sodium caseinate to enhance lignocellulosic hydrolysis and cellulase recovery
Using highly recyclable sodium caseinate to enhance lignocellulosic hydrolysis and cellulase recovery
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使用高度可回收的酪蛋白酸钠增强木质纤维素水解和纤维素酶回收
DOI：
10.1016/j.biortech.2020.122974
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发表时间：
2020-05-01
影响因子：
11.4
通讯作者：
Qiu, Xueqing
中科院分区：
文献类型：
--
作者：
Cai, Cheng;Bao, Yu;Qiu, Xueqing
大多数能够增强木质纤维素酶解的添加剂都是石油基的，不易回收，生物降解性较差。在这项工作中，高度可回收和可生物降解的酪蛋白酸钠（SC）被用来增强木质纤维素水解与改善纤维素酶的可回收性。当pH值从5.5降至4.8时，96%以上的SC可从溶液中沉淀并回收。在pH = 5.5、Eu-DA负载量为10wt%的条件下，添加SC可使Eu-DA的酶消化率从39.5%提高到78.2%，并使72 h酶解液中纤维素酶含量从15.2%提高到60.0%，有利于新鲜底物对纤维素酶的再吸附回收。通过多次再吸附，SC的应用不仅使Eu-DA的糖产量增加了95.5%，而且使纤维素酶负载降低了40%。
Most additives that capable of enhancing enzymatic hydrolysis of lignocellulose are petroleum-based, which are not easy to recycle with poor biodegradability. In this work, highly recyclable and biodegradable sodium caseinate (SC) was used to enhance lignocellulosic hydrolysis with improved cellulase recyclability. When the pH decreased from 5.5 to 4.8, more than 96% SC could be precipitated from the solution and recovered. Adding SC increased enzymatic digestibility of dilute acid pretreated eucalyptus (Eu-DA) from 39.5% to 78.2% under Eu-DA loading of 10 wt% and pH = 5.5, and increase cellulase content in 72 h hydrolysate from only 15.2% of the original to 60.0%, which facilitated the recovery of cellulases through re-adsorption by fresh substrates. With multiple cycles of re-adsorption, application of SC not only increased the sugar yield of Eu-DA by 95.5%, but also reduced cellulase loading by 40%.