Gene expression profile and physiological and biochemical characterization of hexaploid wheat inoculated with Blumeria graminis f. sp tritici
Gene expression profile and physiological and biochemical characterization of hexaploid wheat inoculated with Blumeria graminis f. sp tritici
复制标题
接种Blumeria graminis f的六倍体小麦的基因表达谱及生理生化特征。
DOI：
10.1016/j.pmpp.2015.02.005
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发表时间：
2015-04-01
影响因子：
2.7
通讯作者：
Luo, P. G.
中科院分区：
文献类型：
--
作者：
Ma, L. X.;Zhong, S. F.;Luo, P. G.
小麦(Triticum aestivum L.)白粉病对光合作用和与能量相关的途径有直接影响。中国说，由于小麦白粉病在四川省流行，因此了解PM40介导的小麦白粉病抗性对于了解病原菌之间的相互作用具有重要意义。本研究的目的是了解白粉病病原菌相互作用过程中的生理变化，包括白粉病抗性反应的基因表达、光合作用和叶绿素荧光参数、叶绿素含量和抗氧化活性。以表达PM40的L693和缺失PM40的L1095小麦品系为材料进行了比较分析。从BGT侵染的L693和L1095叶片正向和反向抑制消减杂交文库中分别获得了58个26个表达序列标签(EST)，其中69%的EST与光合作用和能量代谢相关。我们的研究结果表明，与光合作用相关的光合作用、碳代谢和氮代谢是PM40介导小麦白粉病抗性的主要途径，乙酰辅酶A类似基因可能是这些途径中的关键调控因子。本研究为小麦白粉病抗性的生理遗传机制研究提供了新的思路。(C)2015爱思唯尔有限公司。保留所有权利。
Wheat (Triticum aestivum L.) powdery mildew has direct effects on photosynthesis- and energy-related pathways. Because it is prevalent in Sichuan Province, China, knowledge concerning Pm40-mediated wheat powdery mildew resistance is important for understanding hostepathogen interactions. The aim of this study was to understand physiological changes during the hostepathogen interaction, including gene expression, photosynthetic and chlorophyll fluorescence parameters, chlorophyll content, and antioxidant activity, specific to the resistance response to powdery mildew. The Pm40-expressing L693 and Pm40-deficient L1095 wheat lines were employed for comparison analyses. Fifty-eight and 26 expressed sequence tags (ESTs) were obtained from forward and reverse suppression subtractive hybridization cDNA libraries constructed from Bgt-infected L693 and L1095 leaves, respectively, and 69% of the ESTs in the forward library were related to photosynthesis- and energy metabolism-related pathways. Our data indicate that photosynthesis and carbon metabolism as well as nitrogen metabolism related to photosynthesis are the main pathways involved in Pm40-mediated wheat powdery mildew resistance and that an acetyl CoA-like gene might be a key regulatory factor in these pathways. This study provides new insight into the physiological and genetic mechanisms of resistance to wheat powdery mildew. (C) 2015 Elsevier Ltd. All rights reserved.