Recombinant expression of glpK and glpD genes improves the accumulation of shikimic acid in E. coli grown on glycerol
Recombinant expression of glpK and glpD genes improves the accumulation of shikimic acid in E. coli grown on glycerol
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DOI：
10.1007/s11274-014-1753-6
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发表时间：
2014-10
影响因子：
4.1
通讯作者：
Yang Yang-Yang;Chaoqun Yuan;J. Dou;Xiaorong Han;Hui Wang;H. Fang;Changlin Zhou
中科院分区：
文献类型：
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作者：
Yang Yang-Yang;Chaoqun Yuan;J. Dou;Xiaorong Han;Hui Wang;H. Fang;Changlin Zhou
莽草酸 (SA) 是一种工业上重要的手性化合物，用于多种商业应用，由于从植物中分离和昂贵的化学合成而导致的供应不足，增加了开发 SA 合成策略的重要性。在我们之前的研究中，甘油被观察到是大肠杆菌中SA积累的有效碳源。 coliDHPYAAS-T7，其中 PTS 操纵子 (ptsHIcrr) andaroLandaroK 基因失活，tktA、glk、aroE、aroFfbr 和 andaroB 基因过表达。为进一步研究甘油好氧发酵对大肠杆菌中SA积累的影响。 coliBL21(DE3)中glpD、glpK基因和tktA、glk、aroE、aroFfbr、aroB基因同时过表达。结果表明，摇瓶中SA产量比亲本菌株提高了5.6倍，产量提高了5.3倍。结果表明，与glpD和glpK基因过表达相关的甘油有氧发酵增加了甘油通量，导致大肠杆菌中SA积累量更高。大肠杆菌BL21(DE3)-P-DK。
Shikimic acid (SA) is an industrially important chiral compound used in diverse commercial applications, and the insufficient supply by isolation from plants and expensive chemical synthesis of SA has increased the importance of developing strategies for SA synthesis. In our previous studies, glycerol was observed to be an effective carbon source for SA accumulation inE. coliDHPYAAS-T7, where the PTS operon (ptsHIcrr) andaroLandaroKgenes were inactivated, and thetktA,glk,aroE,aroFfbr, andaroBgenes were overexpressed. For further investigation of the effects of glycerol aerobic fermentation on SA accumulation inE. coliBL21(DE3), theglpD,glpKgenes andtktA,glk,aroE,aroFfbr,aroBgenes were overexpressed simultaneously. The results indicated that SA production was increased 5.6-fold, while the yield was increased 5.3-fold over that of parental strain in shake flasks. It is demonstrated that the aerobic fermentation of glycerol associated withglpDandglpKgene overexpression increased glycerol flux, resulting in higher SA accumulation inE. coliBL21(DE3)-P-DK.