Injectable chitosan hyaluronic acid hydrogels for cartilage tissue engineering
Injectable chitosan hyaluronic acid hydrogels for cartilage tissue engineering
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DOI：
10.1016/j.actbio.2012.08.033
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发表时间：
2013-01-01
影响因子：
9.7
通讯作者：
Lee, Min
中科院分区：
文献类型：
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作者：
Park, Hyejin;Choi, Bogyu;Lee, Min
可注射软骨构建体在组织缺损中形成凝胶，在组织工程应用中具有许多优势。本研究以甲基丙烯酸乙二醇壳聚糖（MeGC）和透明质酸（HA）为原料，在可见光下与核黄素光引发剂进行光交联制备了可注射水凝胶。最小辐照时间为40 s，制备出稳定的细胞包封凝胶，包封软骨细胞存活率为87-90%。虽然将辐照时间从40秒增加到600秒，可以显著提高MeGC或MeGC/HA水凝胶的压缩模量，分别达到11或17 kPa，但这些条件使被包裹细胞的活力降低到60-65%。经300 s照射后，包裹在MeGC水凝胶中的大部分软骨细胞保持圆形，在21天的培养周期内细胞存活率高达80-87%。在MeGC水凝胶中掺入透明质酸增加了被包裹软骨细胞的软骨细胞外基质的增殖和沉积。这些发现表明MeGC/HA复合水凝胶具有修复软骨的潜力。(c) 2012材料学报Elsevier Ltd.出版。版权所有。
Injectable cartilaginous constructs that can form gels in tissue defects have many advantages in tissue engineering applications. In this study we created an injectable hydrogel consisting of methacrylated glycol chitosan (MeGC) and hyaluronic acid (HA) by photocrosslinking with a riboflavin photoinitiator under visible light. A minimum irradiation time of 40 s was required to produce stable gels for cell encapsulation with 87-90% encapsulated chondrocyte viability. Although increasing the irradiation time from 40 to 600 s significantly enhanced the compressive modulus of the hydrogels up to 11 or 17 kPa for MeGC or MeGC/HA, respectively, these conditions reduced the encapsulated cell viability to 60-65%. The majority of chondrocytes encapsulated in MeGC hydrogels after 300 s irradiation maintained a rounded shape with a high cell viability of similar to 80-87% over a 21 day culture period. The incorporation of HA in MeGC hydrogels increased the proliferation and deposition of cartilaginous extracellular matrix by encapsulated chondrocytes. These findings demonstrate that MeGC/HA composite hydrogels have the potential for cartilage repair. (c) 2012 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.