Mutations in E coli cistrons affecting adhesion to human cells do not abolish Pap pili fiber formation.
Mutations in E coli cistrons affecting adhesion to human cells do not abolish Pap pili fiber formation.
复制标题
影响与人体细胞粘附的大肠杆菌顺反子的突变不会消除乳头菌毛纤维的形成。
DOI：
10.1002/j.1460-2075.1984.tb01945.x
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发表时间：
1984
期刊：
影响因子：
--
通讯作者：
Uhlin,BE
中科院分区：
文献类型：
--
作者：
Norgren,M;Normark,S;Lark,D;O'Hanley,P;Schoolnik,G;Falkow,S;Svanborg-Edén,C;Båga,M;Uhlin,BE
从致尿路感染大肠埃希菌临床分离株J 96中分离出一个染色体DNA片段，该片段介导Pap（肾盂肾炎相关皮利）皮利形成、甘露糖抗性血凝（MRHA）和与尿路上皮细胞结合，并进行了遗传学研究。对Pap DNA表达的多肽进行分析，检测到大量多肽，其分子量范围为mol.重量从13000到81000道尔顿。四个相应顺反子的基因顺序和转录方向为：13 000（papB）19 500（papA，Pap菌毛亚基的结构基因），81 000（papC）和28 500（papD）。lacZ-papA基因融合体的分析位于克隆DNA内papA上游的启动子。转座子Tn 5插入这四个顺反子中的任何一个都减少或消除了巴氏皮利形成。在papD的远端区域中鉴定了许多转座子Tn 5突变，其以可识别的皮利结构的形式在细胞表面上表达正常水平的papA蛋白，但不凝集人红细胞或粘附于尿路上皮细胞。该区域表达15 000、24 000、26 000和35 000道尔顿的多肽。这一发现表明，巴氏皮利的形成和结合特性可以在遗传上分离。
A chromosomal DNA fragment which mediates Pap (pili associated with pyelonephritis) pili formation, mannose‐resistant hemagglutination (MRHA) and binding to uroepithelial cells has been isolated from the uropathogenic Escherichia coli clinical isolate J96, and genetically studied. Analysis of polypeptides expressed by the Pap DNA led to detection of a number of polypeptides ranging in mol. wt. from 13 000 to 81 000 daltons. The gene order and transcriptional orientation for four of the corresponding cistrons was: 13 000 (papB) 19 500 (papA, structural gene for the Pap pilus subunit), 81 000 (papC) and 28 500 (papD). Analyses of a lacZ‐ papA gene fusion located a promoter upstream from papA within the cloned DNA. Transposon Tn5 insertions in any of these four cistrons decreased or eliminated Pap pili formation. A number of transposon Tn5 mutations were identified in a region distal to papD that expressed normal levels of the papA protein on the cell surface in the form of recognizable pili structures but did not agglutinate human erythrocytes or adhere to uroepithelial cells. This region expressed polypeptides of 15 000, 24 000, 26 000 and 35 000 daltons. This finding shows that Pap pili formation and binding properties can be genetically dissociated.