Analysis of cytokine expression in muscle in inflammatory myopathies, Duchenne dystrophy, and non-weak controls
Analysis of cytokine expression in muscle in inflammatory myopathies, Duchenne dystrophy, and non-weak controls
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DOI：
10.1016/0165-5728(95)00122-0
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发表时间：
1995-12-01
影响因子：
3.3
通讯作者：
Engel, AG
中科院分区：
文献类型：
--
作者：
Lundberg, I;Brengman, JM;Engel, AG
我们研究了15例炎症性肌病（IM）（多发性肌炎、包涵体肌炎和皮肌炎各5例）和10例对照（Duchenne营养不良5例和非虚弱组5例）肌肉中细胞因子mRNA的表达谱。主要来源于T细胞的细胞因子(白细胞介素(IL)-2、IL-4、IL-5和干扰素- γ (ifn - γ)的表达，主要来源于巨噬细胞的细胞因子（IL-1、IL-6和肿瘤坏死因子- α (tnf - α)）的表达，以及主要来源于T细胞或巨噬细胞的细胞因子（粒细胞-巨噬细胞集落刺激因子（GM-CSF）和转化生长因子- β 1 （tgf - β 1）和tgf - β 2）的表达，通过逆转录- pcr方法进行监测。T细胞细胞因子mrna IL-2、IL-5和ifn - γ的表达普遍较弱或不一致。IL-4 mRNA的表达在多发性肌炎中一直是中强的，但在其他IMs中普遍较弱或不存在。在所有病例中，巨噬细胞IL-1 α和IL-1 β mrna的表达均较弱或缺失。15例IM患者中有12例观察到tnf - α mRNA表达变化，10例对照中有5例观察到tnf - α mRNA表达微弱或弱。15例IM中有12例检测到非常强的GM-CSF表达，但仅在增强PCR上检测到，而在对照组中没有检测到。与上述几种细胞因子mRNA的可变表达相反，所有IM标本都强烈表达tgf - β 1 mRNA， 15个标本中有12个强烈表达tgf - β 2 mRNA。因此，除了IL-4在多发性肌炎中的表达外，不同类型的IMs中存在类似的细胞因子mRNA表达模式。此外，这种模式类似于在非弱对照和DD对照中检测到的，尽管在非弱对照中表达通常较弱。研究结果表明，在IM肌肉中，T细胞持续分泌细胞因子或巨噬细胞持续分泌IL-1不是免疫效应反应运作的先决条件，肌肉可能不是持续致敏的部位。
We investigated the profiles of cytokine mRNA expression in muscle in 15 cases of inflammatory myopathy (IM) (5 each of polymyositis, inclusion body myositis, and dermatomyositis) and in 10 controls (5 of Duchenne dystrophy and 5 non-weak subjects). Expressions of the predominantly T cell-derived cytokines (interleukin (IL)-2, IL-4, IL-5, and interferon-gamma (IFN-gamma), of the predominantly macrophage-derived cytokines (IL-1, IL-6, and tumor necrosis factor-alpha (TNF-alpha)), as well as cytokines that can be of either T cell or macrophage origin (granulocyte-macrophage colony stimulating factor (GM-CSF) and transforming growth factor beta 1 (TGF-beta 1) and TGF-beta 2), were monitored by the reverse transcriptase-PCR method. The expression of T cell cytokine mRNAs for IL-2, IL-5, and IFN-gamma was generally weak or inconsistent. IL-4 mRNA expression was consistently moderate to strong in polymyositis but generally weak or absent in the other IMs. The expression of macrophage cytokine mRNAs for IL-1 alpha and IL-1 beta was weak or absent in all cases. Variable TNF-alpha mRNA expression was observed in 12 of 15 IM cases and faint or weak expression in 5 of 10 controls. Very strong GM-CSF expression was detected, but only on boosted PCR, in 12 of 15 cases of IM but in none of the controls. IL-6 was expressed only weakly or inconsistently, In contrast to the variable expression of several of the above mentioned cytokine mRNAs, all IM specimens strongly expressed TGF-beta 1 mRNA and 12 of 15 strongly expressed TGF-beta 2 mRNA. Thus, with the exception of IL-4 expression in polymyositis, a similar pattern of cytokine mRNA expression exists in the different types of IMs. Moreover, this pattern resembles that detected in non-weak and DD controls, although expression is generally weaker in the non-weak controls. The findings suggest that in IM muscle a sustained secretion of cytokines by T cells or of IL-1 by macrophages is not a prerequisite for operation of the immune effector response and that muscle may not be the site of ongoing sensitization.