Spatially resolved detection of antibody-antigen reaction on solid/liquid interface using total internal reflection excited antigen fluorescence and charge-coupled device detection.
Spatially resolved detection of antibody-antigen reaction on solid/liquid interface using total internal reflection excited antigen fluorescence and charge-coupled device detection.
复制标题
使用全内反射激发抗原荧光和电荷耦合装置检测对固/液界面上的抗体-抗原反应进行空间分辨检测。
DOI：
10.1016/0956-5663(90)85002-t
复制
发表时间：
1990
影响因子：
12.6
通讯作者：
Andrade,JD
中科院分区：
文献类型：
--
作者：
Hlady,V;Lin,JN;Andrade,JD
Spatially-resolved detection of antibody—antigen reactions at the solid/liquid interface was investigated by total internal reflection excited fluorescence from large area flat surfaces. Anti-HSA immunoglobulin G(IgG) antibody was immobilized at four spatially distinct spots. Binding of fluorescein-labeled human serum albumin (HSA) from the solution to immobilized antibody was detected by a cooled charge-coupled device (CCD) as a change in the fluorescence intensity. A two-dimensional representation of the fluorescence was obtained during the binding reaction time of 25 mins. The contributions from bound and free antigen to the total signal were evaluated. The influence of the scattered excitation light and the normalization of fluorescence signal with respect to the two-dimensional incident light intensity distribution are discussed.
