Crosslinking porcine aortic valve by radical polymerization for the preparation of BHVs with improved cytocompatibility, mild immune response, and reduced calcification
Crosslinking porcine aortic valve by radical polymerization for the preparation of BHVs with improved cytocompatibility, mild immune response, and reduced calcification
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DOI：
10.1177/0885328220984066
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发表时间：
2021-01-21
影响因子：
2.9
通讯作者：
Wang, Yunbing
中科院分区：
文献类型：
--
作者：
Xu, Liangpeng;Yang, Fan;Wang, Yunbing
由于瓣膜狭窄或返流，每年有100多万例人工心脏瓣膜移植手术。其中，生物人工心脏瓣膜(BHV)由于不需要终生抗凝而得到越来越多的应用。几乎所有的商业BHV都是用戊二醛(过剩)处理的。由于超载处理的BHV容易发生钙化和结构退化，其耐久性大大降低，使用寿命仅为12-15年。猪主动脉瓣(PAV)的生理结构和力学性能更接近于人的心脏瓣膜，因此本研究以PAV为模型，探讨自由基聚合交联法制备的PAV的综合性能。我们发现，自由基聚合交联法处理的PAV具有与GLUT处理的PAV相似的ECM稳定性和双轴力学性能。然而，在体外细胞实验中，自由基聚合交联型PAV表现出更好的细胞相容性和内皮化能力，在大鼠皮下实验中显示出比GLUT处理的PAV更好的抗钙化能力和免疫应答。综上所述，异种组织非戊二醛固定的一种新的交联法有望用于制备BHV。
Over one million artificial heart valve transplantations are performed each year due to valvular stenosis or regurgitation. Among them, bioprosthetic heart valves (BHVs) are increasingly being used because of the absence of the need for lifelong anticoagulation. Almost all of the commercial BHVs are treated with Glutaraldehyde (GLUT). As GLUT-treated BHVs are prone to calcification and structural degradation, their durability is greatly reduced with a service life of only 12-15 years. The physiological structure and mechanical properties of the porcine aortic valve (PAV) are closer to that of a human heart valve, so in this study, PAV is used as the model to explore the comprehensive properties of the prepared BHVs by radical polymerization crosslinking method. We found that PAV treated by radical polymerization crosslinking method showed similar ECM stability and biaxial mechanical properties with GLUT-treated PAV. However, radical polymerization crosslinked PAV exhibited better cytocompatibility and endothelialization potential in vitro cell experiment as better anticalcification potential and reduced immune response than GLUT-treated PAV through subcutaneous animal experiments in rats. To conclude, a novel crosslinking method of non-glutaraldehyde fixation of xenogeneic tissues for the preparation of BHVs is expected.