Extensive Astrocyte Infection Is Prominent in Human Immunodeficiency Virus-Associated Dementia
Extensive Astrocyte Infection Is Prominent in Human Immunodeficiency Virus-Associated Dementia
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DOI：
10.1002/ana.21697
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发表时间：
2009-08-01
影响因子：
11.2
通讯作者：
Gorry, Paul R.
中科院分区：
文献类型：
--
作者：
Churchill, Melissa J.;Wesselingh, Steven L.;Gorry, Paul R.
星形胶质细胞感染人类免疫缺陷病毒（HIV）被认为是罕见的，因此星形胶质细胞被认为在HIV神经发病机制中起次要作用。通过结合双重免疫组织化学，激光捕获显微切割，和高灵敏度的多重聚合酶链反应，以检测体内单个星形胶质细胞中的HIV DNA，我们发现，星形胶质细胞感染是广泛的受试者与HIV相关的痴呆，发生在高达19%的GFAP+细胞。此外，星形胶质细胞感染频率与神经病理学变化的严重程度和血管周围巨噬细胞的接近程度相关。我们的数据表明，星形胶质细胞可以广泛感染艾滋病毒，并建议艾滋病毒感染的星形胶质细胞在艾滋病毒神经发病机制的重要作用。
Astrocyte infection with human immunodeficiency virus (HIV) is considered rare, so astrocytes are thought to play a secondary role in HIV neuropathogenesis. By combining double immunohistochemistry, laser capture microdissection, and highly sensitive multiplexed polymerase chain reaction to detect HIV DNA in single astrocytes in vivo, we showed that astrocyte infection is extensive in subjects with HIV-associated dementia, occurring in up to 19% of GFAP+ cells. In addition, astrocyte infection frequency correlated with the severity of neuropathological changes and proximity to perivascular macrophages. Our data indicate that astrocytes can be extensively infected with HIV, and suggest an important role for HIV-infected astrocytes in HIV neuropathogenesis.