Doped tricalcium phosphate bone tissue engineering scaffolds using sucrose as template and microwave sintering: enhancement of mechanical and biological properties
Doped tricalcium phosphate bone tissue engineering scaffolds using sucrose as template and microwave sintering: enhancement of mechanical and biological properties
复制标题
DOI：
10.1016/j.msec.2017.03.167
复制
发表时间：
2017-09-01
影响因子：
7.9
通讯作者：
Bose, Susmita
中科院分区：
文献类型：
--
作者：
Ke, Dongxu;Bose, Susmita
β-磷酸三钙（β-TCP）是在整形外科和牙科应用中广泛使用的生物相容性陶瓷。然而，其骨诱导性和机械性能仍需要改进。本研究采用蔗糖热分解法制备了多孔β-TCP和MgO/ZnO-TCP支架。由于MgO和ZnO的稳定作用，只能通过添加MgO和ZnO来制备无裂纹的圆柱形支架。通过在1250 ℃下常规烧结后使用25wt%蔗糖制备密度为6139 +/-0.66%、估计孔径为200 μ m和压缩强度为24.96 +/- 3.07 MPa的多孔MgO/ZnO-TCP支架。微波烧结进一步增加了压缩强度至37.94 +/- 6.70 MPa，但它降低了开放互连孔隙率至8.74 +/-138%。此外，聚己内酯（PCL）的掺入增加了2236 +/-3.22%的韧性，同时将其压缩强度保持在25.45 +/-2.21MPa。将人成骨细胞接种于支架上，观察MgO/ZnO和PCL对β-TCP生物学性能的影响。MgO/ZnO和PCL均提高了β-TCP的骨诱导性。PCL也减少成骨细胞凋亡，由于其特殊的表面化学。这种新型的多孔MgO/ZnO-TCP支架与PCL相比具有更好的力学性能和生物学性能，在骨组织工程中具有很大的应用潜力。(C)2017由Elsevier B. V.出版
beta-tricalcium phosphate (beta-TCP) is a widely used biocompatible ceramic in orthopedic and dental applications. However, its osteoinductivity and mechanical properties still require improvements. In this study, porous beta TCP and MgO/ZnO-TCP scaffolds were prepared by the thermal decomposition of sucrose. Crack-free cylindrical scaffolds could only be prepared with the addition of MgO and ZnO due to their stabilization effects. Porous MgO/ZnO-TCP scaffolds with a density of 6139 +/- 0.66%, an estimated pore size of 200 mu m and a compressive strength of 24.96 +/- 3.07 MPa were prepared by using 25 wt% sucrose after conventional sintering at 1250 degrees C. Microwave sintering further increased the compressive strength to 37.94 +/- 6.70 MPa, but it decreased the open interconnected porosity to 8.74 +/- 138%. In addition, the incorporation of polycaprolactone (PCL) increased 2236 +/- 3.22% of toughness while maintaining its compressive strength at 25.45 +/- 2.21 MPa. Human osteoblast cell line was seeded on scaffolds to evaluate the effects of MgO/ZnO and PCL on the biological property of (beta-TCP in vitro. Both MgO/ZnO and PCL improved osteoinductivity of beta-TCP. PCL also decreased osteoblastic apoptosis due to its particular surface chemistry. This novel porous MgO/ZnO-TCP scaffold with PCL shows improved mechanical and biological properties, which has great potential in bone tissue engineering applications. (C) 2017 Published by Elsevier B.V.