Microtubule translocation caused by three subspecies of inner‐arm dynein from Chlamydomonas flagella
Microtubule translocation caused by three subspecies of inner‐arm dynein from Chlamydomonas flagella
复制标题
衣藻鞭毛内臂动力蛋白三个亚种引起的微管易位
DOI：
10.1016/0014-5793(90)80243-c
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发表时间：
1990
期刊：
影响因子：
3.5
通讯作者：
R. Kamiya
中科院分区：
文献类型：
--
作者：
O. Kagami;S. Takada;R. Kamiya
为了帮助理解内臂动力蛋白在小脑运动中的功能，检测了来自衣原体外臂缺失突变体（odal）的动力蛋白样品在体外易位微管的能力。含有内臂动力蛋白的轴索酮的高盐提取物通过离子交换色谱分离成7个具有ATP酶活性的峰级分。其中，三个馏分含有不同的动力蛋白重链易位微管。最大速度均在3 ~ 5之间，与微管在解聚轴突中的滑动速度相当。
To help understand the function of inner‐arm dynein in nagellar motility, dynein samples from an outer arm‐missing mutant ofChlamydomonas (odal)were examined for the ability to translocate microtubules in vitro. High‐salt extract ofaxonemes containing inner‐arm dynein was separated by ion‐exchange chromatography into 7 peak fractions with ATPase activities. Of these, three fractions containing different sets of dynein heavy chains translocated microtubules. The maximal velocities were all between 3 and 5 , which were comparable to the microtubule sliding rate in disintegratingodaaxonemes.