Bringing Light to Transcription: The Optogenetics Repertoire.
Bringing Light to Transcription: The Optogenetics Repertoire.
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DOI：
10.3389/fgene.2018.00518
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发表时间：
2018
影响因子：
3.7
通讯作者：
Rincon-Limas DE
中科院分区：
文献类型：
--
作者：
de Mena L;Rizk P;Rincon-Limas DE
操纵外源基因在生物体特定区域表达的能力，深刻地改变了我们研究正常发育和疾病中生物分子过程的方式。不幸的是，大多数经典的诱导系统缺乏良好的空间和时间的准确性，从而限制了强烈依赖于时间，激活持续时间，或细胞定位的分子事件的研究。通过利用对特定波长作出反应的基因工程光敏蛋白，我们现在可以以前所未有的精度对许多分子活动进行急性控制。在这篇综述中，我们提出了一个全面的细分目前所有的光遗传学系统，适用于调节基因表达的单细胞和多细胞生物。我们专注于这些不同的工具的优点和缺点，并讨论当前和未来的挑战，在成功的翻译到更复杂的生物体。
The ability to manipulate expression of exogenous genes in particular regions of living organisms has profoundly transformed the way we study biomolecular processes involved in both normal development and disease. Unfortunately, most of the classical inducible systems lack fine spatial and temporal accuracy, thereby limiting the study of molecular events that strongly depend on time, duration of activation, or cellular localization. By exploiting genetically engineered photo sensing proteins that respond to specific wavelengths, we can now provide acute control of numerous molecular activities with unprecedented precision. In this review, we present a comprehensive breakdown of all of the current optogenetic systems adapted to regulate gene expression in both unicellular and multicellular organisms. We focus on the advantages and disadvantages of these different tools and discuss current and future challenges in the successful translation to more complex organisms.