The role of alternative mRNA splicing in generating heterogeneity within the Anopheles gambiae class I glutathione S-transferase family
The role of alternative mRNA splicing in generating heterogeneity within the Anopheles gambiae class I glutathione S-transferase family
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DOI：
10.1073/pnas.95.24.14284
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发表时间：
1998-11-24
影响因子：
11.1
通讯作者：
Hemingway, J
中科院分区：
文献类型：
--
作者：
Ranson, H;Collins, F;Hemingway, J
大劣按蚊的谷胱甘肽S转移酶(GSTs)是由一个复杂的基因家族编码的。我们描述了这个家族的三个成员的基因组组织，它们以不同的方向顺序排列在染色体上。其中一个基因aggst1-2是无内含子的，已被描述。相反，报告的两个Garnbiae GST基因(aggst1α和aggst1 beta)被内含子打断。基因aggst1α包含Fire编码外显子，这些外显子交替剪接产生四个成熟的GST转录本，每个外显子包含一个共同的5‘外显子，编码GST蛋白的N端，与四个不同的3’外显子之一拼接，编码羧基末端。Aggst1α的所有四个可选转录本都在大斑潜蝇幼虫、蛹和成虫中表达。我们报道了选择性RNA剪接参与产生多种功能的GST转录本。在成年蚊子中检测到aggst1β基因的cdna，表明该GST基因被活跃地转录。3个GST基因转录的6个cDNA在核苷酸水平上的相似性百分比在49.5%~83.1%之间。
The class I glutathione S-transferases (GSTs) of Anopheles garnbiae are encoded by a complex gene family. We describe the genomic organization of three members of this family, which are sequentially arranged on the chromosome in divergent orientations. One of these genes, aggst1-2, is intronless and has been described. In contrast, the two A. garnbiae GST genes (aggst1 alpha and aggst1 beta) reported within are interrupted by introns. The gene aggst1 alpha contains fire coding exons that are alternatively spliced to produce four mature GST transcripts, each of which contains a common 5' exon encoding the N termini of the GST protein spliced to one of four distinct 3' exons encoding the carboxyl termini. All four of the alternative transcripts of aggst1 alpha are expressed in A. garnbiae larvae, pupae, and adults. We report on the involvement of alternative RNA splicing in generating multiple functional GST transcripts. A cDNA from the aggst1 beta gene was detected in adult mosquitoes, demonstrating that this GST gene is actively transcribed. The percentage similarity of the six cDNAs transcribed from the three GST genes range from 49.5% to 83.1% at the nucleotide level.