Phosphorylation of linker histone H1 regulates gene expression in vivo by mimicking H1 removal
Phosphorylation of linker histone H1 regulates gene expression in vivo by mimicking H1 removal
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DOI：
10.1016/s1097-2765(00)80215-4
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发表时间：
1999-10-01
期刊：
影响因子：
16
通讯作者：
Gorovsky, MA
中科院分区：
文献类型：
--
作者：
Dou, YL;Mizzen, CA;Gorovsky, MA
通过基因替换创建了两种四膜虫菌株。其中一个包含 H1，所有磷酸化位点都突变为丙氨酸，从而阻止磷酸化。另一个将这些位点改为谷氨酸，模仿完全磷酸化的状态。在这两种菌株中，整体基因表达均未发生可检测到的变化。相反，H1 磷酸化激活或抑制特定基因的方式与敲除 H1 编码基因的效果非常相似。这些研究证明了 H1 磷酸化在体内转录调节中的作用，并表明它通过模仿 H1 的部分去除来发挥作用。
Two Tetrahymena strains were created by gene replacement. One contained H1 with all phosphorylation sites mutated to alanine, preventing phosphorylation. The other had these sites changed to glutamic acid, mimicking the fully phosphorylated state. Global gene expression was not detectably changed in either strain. Instead, H1 phosphorylation activated or repressed specific genes in a manner that was remarkably similar to the effects of knocking out the gene encoding H1. These studies demonstrate a role for H1 phosphorylation in the regulation of transcription in vivo and suggest that it acts by mimicking the partial removal of H1.