Enterococcus faecalis pheromone binding protein, PrgZ, recruits a chromosomal oligopeptide permease system to import sex pheromone cCF10 for induction of conjugation
Enterococcus faecalis pheromone binding protein, PrgZ, recruits a chromosomal oligopeptide permease system to import sex pheromone cCF10 for induction of conjugation
复制标题
DOI：
10.1073/pnas.93.1.260
复制
发表时间：
1996-01-09
影响因子：
11.1
通讯作者：
Dunny, GM
中科院分区：
文献类型：
--
作者：
Leonard, BAB;Podbielski, A;Dunny, GM
粪肠球菌（Enterococcus faecalis）供体细胞对pCF 10质粒的接合转移是对受体细胞分泌的肽类性信息素cCF 10的应答。faecalisOpp操纵子消除了生理浓度信息素的反应，亲和层析实验证明了信息素与几种推定的细胞内调节分子的相互作用，包括缀合功能正调节所需的RNA分子，这些数据表明，信息素信号的处理涉及PrgZ对染色体Opp系统的募集，并且信号传导通过内化的具有细胞内效应物的信息素
Conjugative transfer of the plasmid pCF10 by Enterococcus faecalis donor cells occurs in response to a peptide sex pheromone, cCF10, secreted by recipients, The plasmid-encoded cCF10 binding protein, PrgZ, is similar in sequence to binding proteins (OppAs) encoded bg oligopeptide permease (opp) operons, Mutation of prgZ decreased the sensitivity of donor cells to pheromone, whereas inactivation of the chromosomal E. faecalis opp operon abolished response at physiological concentrations of pheromone, Affinity chromatography experiments demonstrated the interaction of the pheromone with several putative intracellular regulatory molecules, including an RNA molecule required for positive regulation of conjugation functions, These data suggest that processing of the pheromone signal involves recruitment of a chromosomal Opp system by PrgZ and that signaling occurs by direct interaction of internalized pheromone with intracellular effecters.