Increased Expression of Hypoxia-inducible Factor-1α and Vascular Endothelial Growth Factor Associated With Glomerulation Formation in Patients With Interstitial Cystitis
Increased Expression of Hypoxia-inducible Factor-1α and Vascular Endothelial Growth Factor Associated With Glomerulation Formation in Patients With Interstitial Cystitis
复制标题
DOI：
10.1016/j.urology.2011.05.050
复制
发表时间：
2011-10-01
期刊：
影响因子：
2.1
通讯作者：
Lee, Ming-Huei
中科院分区：
文献类型：
--
作者：
Lee, Jane-Dar;Lee, Ming-Huei
目的通过检测间质性膀胱炎（IC）患者膀胱组织中缺氧诱导因子-1（HIF-1）和血管内皮生长因子（VEGF）的表达，探讨IC患者膀胱组织中是否存在缺氧。先前的研究已经报道了IC患者的膀胱灌注减少。缺氧诱导血管内皮生长因子（VEGF）的过度表达，这已被报道与IC患者肾小球的形成。方法研究组包括32例IC患者，对照组包括8名志愿者。我们从两组中获得膀胱活检组织，并通过免疫印迹和免疫组化以及双重免疫荧光染色研究HIF-1 α和VEGF蛋白的表达。结果免疫印迹和免疫组化结果显示，与对照组相比，实验组HIF-1 α和VEGF蛋白表达明显增加。研究组中HIF-1 α和VEGF蛋白的相对强度分别为60.60 +/- 7.81和43.60 +/- 5.37，对照组中分别为26.20 +/- 4.72和20.25 +/- 1.45。VEGF在研究组活检组织中的过度表达是特别明显的伞细胞检查共聚焦microscopic.CONCLUSION我们的研究结果确定增加HIF-1 α在膀胱组织中的表达和过度表达的VEGF在伞细胞从IC患者。这些事件可能与IC膀胱水扩张期间的肾小球形成有关。因此，这些分子研究结果可以提供高压氧应用于IC患者的治疗机制。泌尿学78：971.e11-971.e15，2011年。(C)2011 Elsevier Inc.
OBJECTIVE To examine whether hypoxia occurs in the bladders of patients with interstitial cystitis (IC) by monitoring the expression of hypoxia-inducible factor-1 (HIF-1) and VEGF. Previous studies have reported that bladder perfusion is decreased in patients with IC. Hypoxia induces overexpression of vascular endothelial growth factor (VEGF), which has been reported to be associated with the formation of glomerulations in patients with IC.METHODS The study group consisted of 32 patients with IC, and the control group consisted of 8 volunteers. We obtained bladder biopsies from both groups and studied the expression of HIF-1 alpha and VEGF proteins by immunoblotting and immunohistochemical and double immunofluorescent staining. Data were analyzed using the Mann-Whitney U test.RESULTS Immunoblotting and immunostaining revealed that the expression of HIF-1 alpha and VEGF proteins was increased in the study group compared with the control group. The relative intensities of HIF-1 alpha and VEGF proteins were 60.60 +/- 7.81 and 43.60 +/- 5.37 in the study group and 26.20 +/- 4.72 and 20.25 +/- 1.45 in the control group, respectively. The overexpression of VEGF in study group biopsies was particularly evident in umbrella cells examined by confocal microscopy.CONCLUSION Our findings identified increased expression of HIF-1 alpha in bladder tissue and overexpression of VEGF in umbrella cells from patients with IC. These events may be associated with glomerulation formation during hydrodistention in IC bladders. Thus, these molecular findings could offer the therapeutic mechanism for hyperbaric oxygenation application to patients with IC. UROLOGY 78: 971.e11-971.e15, 2011. (C) 2011 Elsevier Inc.