Cytokines, chemokines and soluble adhesion molecules in aqueous humor of children with uveitis
Cytokines, chemokines and soluble adhesion molecules in aqueous humor of children with uveitis
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DOI：
10.1016/j.exer.2007.06.011
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发表时间：
2007-10-01
影响因子：
3.4
通讯作者：
de Boer, Joke H.
中科院分区：
文献类型：
--
作者：
Sijssens, Karen M.;Rijkers, Ger T.;de Boer, Joke H.
儿童葡萄膜炎是一种威胁视力的疾病，并发症发生率超过75%。尽管进行了广泛的研究，但葡萄膜炎的病因仍不清楚，尽管现在普遍认为葡萄膜炎是一种 T 细胞介导的疾病。本研究的目的是调查葡萄膜炎儿童眼房水 (AqH) 中细胞因子、趋化细胞因子（趋化因子）和可溶性粘附分子的概况，以确定控制眼睛免疫反应的因素。在这项临床实验室研究中，我们通过多重免疫分析分析了 25 名患有葡萄膜炎的儿童和 6 名未患葡萄膜炎的儿童的 AqH 中的 16 种免疫介质。与对照组相比，葡萄膜炎儿童的 AqH 中白介素 2 (IL-2)、IL-6、IL-10、IL-13、IL-18、干扰素 γ、肿瘤坏死因子 α、可溶性细胞间粘附分子 1、RANTES、IL-8 和干扰素诱导的 10-kDa 蛋白水平升高。 IL-1β、IL-4、IL-12 p-70、可溶性血管细胞粘附分子1和嗜酸细胞趋化因子没有发现显着差异。与活动性葡萄膜炎（诊断）样本相比，安静期葡萄膜炎（手术）样本中的 IL-10 和 IL-8 水平较低；与未接受 MTX 治疗的患者样本相比，接受甲氨蝶呤 (MTX) 治疗的患者样本中的 IL-10 和 IL-8 水平较低。葡萄膜炎诊断后前 3 个月采集的样本中 IL-10 的水平也低于疾病过程后期采集的样本。使用或不使用局部或全身（围手术期和长期）皮质类固醇治疗的患者之间没有发现显着差异。总之，在患有葡萄膜炎的儿童中，无论活动性还是非活动性炎症，AqH 中多种眼内细胞因子、趋化因子和可溶性粘附分子都会增加。葡萄膜炎儿童AqH中IL-8和IL-10水平是否与葡萄膜炎活动性、病程的早期或晚期以及MTX全身治疗相关，需要在更大的研究人群中进一步研究。 (C) 2007 Elsevier Ltd. 保留所有权利。
Uveitis in childhood is a visual threatening disease with a complication rate of more than 75%. Despite extensive research, the etiology of uveitis is still unclear although the general opinion is now that uveitis is a T-cell mediated disease. The purpose of this study was to investigate the profile of cytokines, chemotactic cytokines (chemokines) and soluble adhesion molecules in the aqueous humor (AqH) of children with uveitis in order to identify the factors that control the immune response in the eye. In this clinical laboratory investigation we analyzed, with a multiplex immunoassay, 16 immune mediators in the AqH of 25 children with uveitis and 6 children without uveitis. Increased levels of interleukin-2 (IL-2), IL-6, IL-10, IL-13, IL-18, interferon-gamma, tumor necrosis factor-alpha, soluble intercellular adhesion molecule-1, RANTES, IL-8 and interferon-inducible 10-kDa protein were found in the AqH of children with uveitis compared with controls. No significant differences were found for IL-1 beta, IL-4, IL-12 p-70, soluble vascular cell adhesion molecule 1 and Eotaxin. Lower levels of IL-10 and IL-8 were found in quiet stage uveitis (surgical) samples compared with active uveitis (diagnostic) samples and in samples of patients treated with methotrexate (MTX) compared with samples of patients not treated with MTX. Lower levels of IL-10 were as well found in samples taken during the first 3 months after the diagnosis of uveitis than samples taken later during the disease process. No significant differences were found between patients treated with or without topical or systemic (perioperative and long term) corticosteroids. In conclusion, in children with uveitis, multiple intraocular cytokines, chemokines and soluble adhesion molecules are increased in the AqH regardless of active or inactive inflammation. Whether the IL-8 and IL-10 levels in AqH of children with uveitis are correlated with uveitis activity, early or late phase of the course of the disease and systemic treatment with MTX needs further investigation in a bigger study population. (C) 2007 Elsevier Ltd. All rights reserved.