The amount of calcium-deficient hexagonal hydroxyapatite in aortic valves is influenced by gender and associated with genetic polymorphisms in patients with severe calcific aortic stenosis
The amount of calcium-deficient hexagonal hydroxyapatite in aortic valves is influenced by gender and associated with genetic polymorphisms in patients with severe calcific aortic stenosis
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DOI：
10.1016/j.ehj.2003.09.006
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发表时间：
2004-03-01
影响因子：
39.3
通讯作者：
Hoffmann, R
中科院分区：
文献类型：
--
作者：
Ortlepp, JR;Schmitz, F;Hoffmann, R
目的探讨心血管危险因素(CRF)、基因多态性、钙化与主动脉瓣狭窄纤维化的关系。方法和结果用原子吸收光谱法测定187例主动脉瓣狭窄切除标本的钙含量。羟脯氨酸含量测定。行左心导管术。检测CRF和白介素10、结缔组织生长因子(CTGF)、趋化因子受体5(CCR5)基因多态性。钙化由缺钙六方羟基磷灰石组成，Ca-(10-x)(HPO4)(X)(PO4)(6-x)(OH)(2-x)，其中0小于或等于x小于或等于1。钙化(五分位数)与主动脉瓣平均倾斜度呈正相关(44+/-14，52+/-17，54+/-16，60+/-15，68+/-19 mm Hg；p<；0.001)。Mate(n=10 1)的钙化程度较高(2 6.1±8.9vs2 0.8±9.2质量%；p<；0.001)，尽管主动脉瓣的平均倾斜度相同(5 617vs5 619 mm Hg；p=0.958)。CRF与钙化程度无关，而白细胞介素10基因-1082、-819和-592与钙化程度显著相关。此外，如果某些等位基因携带者还携带罕见的CCR5或CTGF等位基因，则钙化程度更高。结论主动脉瓣狭窄的钙化由缺钙的六方羟基磷灰石组成。性别和遗传多态对主动脉瓣钙化程度有影响。(C)2003年，由爱思唯尔有限公司代表欧洲心脏病学会出版。
Aims The study evaluated the relationship between Cardiovascular risk factors (CRF), gene polymorphism, calcification and fibrosis of stenotic aortic valves.Methods and results The calcium content of 187 excised stenotic aortic valves was determined using atomic absorption spectroscopy. Hydroxyproline content was quantified. Left-heart catheterization was performed. CRF and genotypes of the interleukin 10, connective tissue growth factor (CTGF) and chemokine receptor 5 (CCR5) polymorphisms were assessed. Calcification consisted of Ca-deficient hexagonal hydroxyapatite, Ca-(10-x)(HPO4)(x)(PO4)(6-x)(OH)(2-x); with 0 less than or equal to x less than or equal to 1. Calcification (quintiles) was positively associated with the mean gradient across the aortic valve (44 +/- 14, 52 +/- 17, 54 +/- 16, 60 +/- 15, 68 +/- 19 mm Hg; p < 0.001). Mates (n = 101) had a higher degree of calcification (26.1 ± 8.9 vs 20.8 ± 9.2 mass%; p < 0.001), despite the same mean gradient across the aortic valve (56 17 vs; 56 19 mm Hg; p = 0.958). CRF were not, whereas interleukin 10 polymorphisms -1082, -819, and -592 were significantly associated with the degree of calcification. Furthermore, if certain allele carriers had additionally the rare CCR5 or CTGF allele the degree of calcification was higher.Conclusion Calcification of stenotic aortic valves consists of Ca-deficient hexagonal hydroxyapatite. Gender and genetic polymorphisms have an impact on the degree of aortic valve calcification. (C) 2003 Published by Elsevier Ltd on behalf of The European Society of Cardiology.