Biosynthesis of proteoglycans by rat granuloma cells cultured in vitro: modulation by gonadotropins, steroid hormones, prostaglandins, and a cyclic nucleotide.
Biosynthesis of proteoglycans by rat granuloma cells cultured in vitro: modulation by gonadotropins, steroid hormones, prostaglandins, and a cyclic nucleotide.
复制标题
体外培养的大鼠肉芽肿细胞的蛋白多糖生物合成：促性腺激素、类固醇激素、前列腺素和环核苷酸的调节。
DOI：
10.1210/endo-109-5-1641
复制
发表时间：
1981
期刊：
影响因子：
4.8
通讯作者：
D. Rodbard
中科院分区：
文献类型：
--
作者：
M. Yanagishita;V. Hascall;D. Rodbard
用5 IU PMS促性腺激素刺激后48 h，从未成熟雌性大鼠中分离大鼠卵巢颗粒细胞，然后维持培养。以[35 S]硫酸盐为前体，研究了绵羊（o）FSH、oLH、hCG、睾酮、17 β-雌二醇、孕酮、野牡丹素E1、E2、F1 α、F2 α、N，O ′-二丁酰cAMP和茶碱对体外颗粒细胞蛋白多糖合成的影响。与对照组相比，oFSH、oLH、hCG、E1和E2、N，O '-二丁酰cAMP、茶碱和睾酮刺激35 S标记的蛋白多糖的产生。17 β-雌二醇、孕酮、和野牡丹素F1 α和F2 α没有显示出刺激作用。两种主要的蛋白聚糖被合成并分泌到培养基中：1）具有大流体动力学大小的群体（在Sepharose CL-2B上Kav = 0.30）;和2）具有相对小大小的群体（在Sepharose CL-2B上Kav = 0.60）。激素的刺激作用完全由较小蛋白聚糖群合成的净增加来解释。这些激素在与颗粒细胞的其他体外生物反应所需的浓度相似的浓度下对蛋白聚糖合成发挥刺激作用。
Rat ovarian granulosa cells were isolated from immature female rats 48 h after stimulation with 5 IU PMS gonadotropin and then maintained in culture. The effects of ovine (o)FSH, oLH, hCG, testosterone, 17 beta-estradiol, progesterone, prostaglandins E1, E2, F1 alpha, F2 alpha, N,O'-dibutyryl cAMP, and theophylline on proteoglycan synthesis by the granulosa cells in vitro were examined using [35S]sulfate as a precursor. oFSH, oLH, hCG, prostaglandins E1 and E2, N,O'-dibutyryl cAMP, theophylline and testosterone stimulated the production of 35S-labeled proteoglycans compared to control cultured. 17 beta-Estradiol, progesterone, and the prostaglandins F1 alpha, and F2 alpha, showed no stimulation. Two major species of proteoglycan are synthesized and secreted into the medium: 1) a population with large hydrodynamic size (Kav = 0.30 on Sepharose CL-2B); and 2) a population with relatively small size (Kav = 0.60 on Sepharose CL-2B). The stimulatory effect of the hormones was accounted for entirely by a net increase in synthesis of the smaller proteoglycan population. The hormones exert their stimulatory effects on proteoglycan synthesis at concentrations similar to those required for other in vitro biological responses of granulosa cells.