PITUITARY ADENYLATE-CYCLASE ACTIVATING PEPTIDE IS A SENSORY NEUROPEPTIDE - IMMUNOCYTOCHEMICAL AND IMMUNOCHEMICAL EVIDENCE
PITUITARY ADENYLATE-CYCLASE ACTIVATING PEPTIDE IS A SENSORY NEUROPEPTIDE - IMMUNOCYTOCHEMICAL AND IMMUNOCHEMICAL EVIDENCE
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DOI：
10.1016/0306-4522(93)90018-b
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发表时间：
1993-12-01
期刊：
影响因子：
3.3
通讯作者：
SUNDLER, F
中科院分区：
文献类型：
--
作者：
MOLLER, K;ZHANG, YZ;SUNDLER, F
腺苷酸环化酶激活肽（PACAP）是一种血管活性肠肽样下丘脑肽，以两种变体存在。PACAP-27和C-末端延伸的PACAP-38。免疫反应性PACAP也已在肠神经系统和呼吸道的神经支配中得到证实。我们研究了PACAP发生在大鼠感觉神经系统的可能性。免疫细胞化学显示，在脊髓背角浅层的许多神经纤维中，在脊神经节和三叉神经节的神经细胞体（其中大部分是小尺寸的）中，以及在鼻、舌、喉气管和膀胱皮肤中的表面上皮细胞附近和内部的神经纤维中，以及在颌下腺导管周围，存在PACAP。在所有的位置，PACAP与降钙素基因相关肽和P物质共存，PACAP免疫反应性纤维和细胞体构成一个亚群的那些存储P物质和/或降钙素基因相关肽。额外的PACAP免疫反应纤维不与上皮细胞似乎缺乏降钙素基因相关肽和P物质。辣椒素治疗降低了密度的PACAP和降钙素基因相关肽/P物质免疫反应纤维在组织检查。这些数据有利于PACAP在初级感觉神经元中的作用。
Pituitary adenylate cyclase activating peptide (PACAP) is a vasoactive intestinal peptide-like hypothalamic peptide occurring as two variants. PACAP-27 and the C-terminally extended PACAP-38. Immunoreactive PACAP has also been demonstrated in the enteric nervous system and in the innervation of the respiratory tract. We have examined the possibility that PACAP occurs in the sensory nervous system of the rat. Immunocytochemistry revealed PACAP in numerous nerve fibres in the superficial layer of the dorsal horns of the spinal cord, in nerve cell bodies (most of them of small size) of the spinal ganglia and trigeminal ganglia and in nerve fibres running close to and within the surface epithelium in the skin of the nose, the tongue, the larynx-trachea, and the urinary bladder as well as around the ducts of the submandibular gland. In all locations, PACAP co-existed with calcitonin gene-related peptide and substance P, the PACAP-immunoreactive fibres and cell bodies constituting a subpopulation of those storing substance P and/or calcitonin gene-related peptide. Additional PACAP-immunoreactive fibres not associated with epithelia seemed to lack calcitonin gene-related peptide and substance P. Capsaicin treatment reduced the density of PACAP- and calcitonin gene-related peptide/substance P-immunoreactive fibres in the tissues examined.On the whole, the immunocytochemical results agreed with those obtained by radioimmunoassay for PACAP and CGRP. The data favour a role for PACAP in primary sensory neurons.