Structure of microbial communities in activated sludge: Potential implications for assessing the biodegradability of chemicals
Structure of microbial communities in activated sludge: Potential implications for assessing the biodegradability of chemicals
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DOI：
10.1006/eesa.2001.2034
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发表时间：
2001-05-01
影响因子：
6.8
通讯作者：
Larson, RJ
中科院分区：
文献类型：
--
作者：
Forney, LJ;Liu, WT;Larson, RJ
用于评估化学品的生物降解性的各种方法通常采用活性污泥作为接种物，因为最终进入环境的化学品通常通过废水排出。可能使生物降解测试的解释复杂化的活性污泥微生物群落的结构和功能的差异可能由废水组成、废水处理厂（WWTP）操作或在收集活性污泥后进行的操作。在本研究中，使用各种方法来表征从日本、欧洲和美国的WWTP新鲜收集的活性污泥中发现的微生物群落结构，以及在收集后连续几周喂食污水或葡萄糖-蛋白胨混合物的污泥中发现的微生物群落结构，比较生物量水平、整个群落基质利用率。（使用Biolog GN和GP板测定）和磷脂脂肪酸（PLFA）谱表明在新鲜收集的活性污泥样品中存在地理和时间差异。此外，在实验室中连续培养5周后，使用葡萄糖-蛋白胨饲料的活性污泥微生物群落的结构发生了显着的变化，这些变化是显而易见的，从整个社区的底物利用率和PLFA配置文件，以及在16 S rDNA基因的配置文件中的差异，从数量优势种群获得变性梯度凝胶电泳和末端限制性片段分析。需要进一步的研究，以更好地确定内和活性污泥之间的变化，从废水处理厂和实验室反应器，并评估这种差异的影响，生物降解性测试的结果。(C)北京：科学出版社.
Various methods used to assess the biodegradability of chemicals often employ activated sludge as an inoculum since chemicals that ultimately enter the environment are often discharged through wastewater, Differences in the structure and function of activated sludge microbial communities that may complicate interpretation of biodegradation tests could arise from differences in wastewater composition, wastewater treatment plant (WWTP) operation, or manipulations done after collection of the activated sludge. In this study, various methods were used to characterize the structure of microbial communities found in freshly collected activated sludge from WWTPs in Japan, Europe, and the United States, as well as sludge that had been continuously fed either sewage or a glucose-peptone mixture for several weeks after collection, Comparisons of biomass levels, whole-community substrate utilization (determined using Biolog GN and GP plates), and phospholipid fatty acid (PLFA) profiles indicated there were both geographical and temporal differences among freshly collected activated sludge samples. Moreover, marked shifts in the structure of activated sludge microbial communities occurred upon continuous cultivation in the laboratory for 5 weeks using a glucose-peptone feed, These shifts were evident from whole-community substrate utilization and PLFA profiles as well as differences in the profiles of 16S rDNA genes from numerically dominant populations obtained by denaturing gradient gel electrophoresis and terminal restriction fragment analyses. Further studies are needed to better define the variability within and between activated sludge from wastewater treatment plants and laboratory reactors and to assess the impact of such differences on the outcome of biodegradability tests. (C) 2001 Academic Press.