Adhesive protein-mediated cross-talk between Candida albicans and Porphyromonas gingivalis in dual species biofilm protects the anaerobic bacterium in unfavorable oxic environment
Adhesive protein-mediated cross-talk between Candida albicans and Porphyromonas gingivalis in dual species biofilm protects the anaerobic bacterium in unfavorable oxic environment
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DOI：
10.1038/s41598-019-40771-8
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发表时间：
2019-03-13
影响因子：
4.6
通讯作者：
Rapala-Kozik, Maria
中科院分区：
文献类型：
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作者：
Bartnicka, Dominika;Karkowska-Kuleta, Justyna;Rapala-Kozik, Maria
口腔中含有不同类型的微生物物种，它们通过广泛的细胞间相互作用和生物膜形成来定植人类宿主。白色念珠菌是一种寄生在粘膜表面的酵母样真菌，也是慢性牙周炎患者牙龈下I区重要的定植菌。值得注意的是，引起牙周病的主要感染因子之一是厌氧细菌-牙龈卟啉单胞菌。在我们的研究中，我们评估了两种病原体在人工表面相互定植时的不同策略，并证实在氧气条件下形成的发达真菌生物膜中存在牙龈假单胞菌的保护环境，真菌细胞主要以丝状形式（即菌丝）生长。真菌与牙龈卟啉卟啉之间的直接物理接触是通过调节真菌细胞表面粘附素Als3和天冬氨酸蛋白酶Sap6和Sap9的基因表达而开始的。真菌表面体的蛋白质组学鉴定也表明Mp65粘附素和“月光”蛋白烯醇化酶参与了与牙龈假单胞菌的相互作用。利用这些细菌的突变菌株，这些菌株在产生牙龈蛋白酶（也含有血凝素结构域的蛋白水解酶）方面有缺陷，这些蛋白质在形成细菌保护生物膜方面的重要作用被清楚地证明了。
The oral cavity contains different types of microbial species that colonize human host via extensive cellto-cell interactions and biofilm formation. Candida albicans-a yeast-like fungus that inhabits mucosal surfaces-is also a significant colonizer of subgingiva I sites in patients with chronic periodontitis. It is notable however that one of the main infectious agents that causes periodontal disease is an anaerobic bacterium-Porphyromonas gingivalis. In our study, we evaluated the different strategies of both pathogens in the mutual colonization of an artificial surface and confirmed that a protective environment existed for P. gingivalis within developed fungal biofilm formed under oxic conditions where fungal cells grow mainly in their filamentous form i.e. hyphae. A direct physical contact between fungi and P. gingivalis was initiated via a modulation of gene expression for the major fungal cell surface adhesin Als3 and the aspartic proteases Sap6 and Sap9. Proteomic identification of the fungal surfaceome suggested also an involvement of the Mp65 adhesin and a "moonlighting" protein, enolase, as partners for the interaction with P. gingivalis. Using mutant strains of these bacteria that are defective in the production of the gingipains-the proteolytic enzymes that also harbor hemagglutinin domains-significant roles of these proteins in the formation of bacteria-protecting biofilm were clearly demonstrated.