Activation of ERK1/2 is required for normal response of isosexual social interactions in male rats
Activation of ERK1/2 is required for normal response of isosexual social interactions in male rats
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DOI：
10.1016/j.brainres.2013.08.046
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发表时间：
2013-11-13
期刊：
影响因子：
2.9
通讯作者：
Li, Chang-Qi
中科院分区：
文献类型：
--
作者：
Cao, Wen-Yu;Xu, Yang;Li, Chang-Qi
以前的研究表明，有丝分裂原活化蛋白激酶（MAPK）通路参与大鼠之间的异性相互作用。然而，很少有研究关注它在同性社会互动中的作用。我们研究了雄性大鼠的同性社会干预行为，使用（i）三腔社会互动盒和（ii）磷酸化细胞外信号调节ldnase 1和2（pERK 1/2）定位在同性行为期间激活的脑区。当面对盒子的社会目标侧与无生命侧时，所有大鼠都更喜欢社会目标侧。在10分钟内，同性的社会互动诱导pERK 1/2在大脑中的表达迅速增加，特别是主要的嗅觉上皮（莫伊）相关的大脑区域。硫酸锌诱导的嗅觉剥夺后，大鼠表现出没有偏好的社会目标或无生命的一方，伴随着MOE相关脑区的pERK 1/2表达下降。此外，为了确定pERK 1/2在同性社会干预行为中的作用，向大鼠腹腔内注射SL 327（30 mg/kg，MAPK激酶抑制剂）。尽管SL 327显著下调了脑pERK 1/2的表达，但实验动物也在社会目标侧花费了更多的时间。这些结果表明：（i）与雄性伴侣短暂的相互作用诱导大鼠脑中ERK 1/2迅速磷酸化。(ii)破坏莫伊的功能可使大鼠的同性社会干预行为消失。(iii)抑制大鼠大脑中磷酸化的ERK 1/2会扰乱它们正常的社会行为。(C)2013爱思唯尔有限公司版权所有。
Previous studies have indicated involvement of the mitogen-activated protein kinase (MAPK) pathway in heterosexual interactions among rats. Very few studies, however, have focused its role in isosexual social interactions. We studied the male rat's isosexual social interactional behavior using (i) the three-chambered social interaction box and (ii) phosphorylated extracellular signal-regulated ldnase 1 and 2 (pERK1/2) to localize the brain regions that are activated during isosexual behavior. When faced with the social target side of the box versus the inanimate side, all rats preferred the social target side. Within 10 min, isosexual social interactions induced a rapid increase in pERK1/2 expression in the brain, especially the main olfactory epithelial (MOE)-related brain regions. After ZnSO4-induced olfactory deprivation, rats showed no preference for either the social target or inanimate side, with a concomitant decrease in pERK1/2 expression in MOE-related brain regions. Additionally, to determine the role of pERK1/2 in isosexual social interactional behavior, rats were injected intraperitoneally with SL327 (30 mg/kg, a MAPK kinase inhibitor). Although SL327 dramatically down-regulated expression of brain pERK1/2, experimental animals also spent significantly more time in the social target side. These results indicate that (i) A brief interacting with a male partner induced rapidly phosphorylated ERK1/2 in the rat's brain. (ii) Destroy the function of MOE abolished the rats' isosexual social interactional behavior. (iii) Suppressed the phosphorylated ERK1/2 in the rats' brain disrupt their normal social behaviour. (C) 2013 Elsevier B.V. All rights reserved.