CLONING OF A TGF-BETA TYPE-I RECEPTOR THAT FORMS A HETEROMERIC COMPLEX WITH THE TGF-BETA TYPE-II RECEPTOR
CLONING OF A TGF-BETA TYPE-I RECEPTOR THAT FORMS A HETEROMERIC COMPLEX WITH THE TGF-BETA TYPE-II RECEPTOR
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DOI：
10.1016/0092-8674(93)90489-d
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发表时间：
1993-11-19
期刊：
影响因子：
64.5
通讯作者：
MIYAZONO, K
中科院分区：
文献类型：
--
作者：
FRANZEN, P;TENDIJKE, P;MIYAZONO, K
获得了编码53 kd丝氨酸/苏氨酸激酶受体的cDNA克隆，其总体结构与转化生长因子β（TGF β）的II型受体相似。 I-125-TGF β 1与用cDNA转染的猪内皮细胞结合，并形成70 kd的交联复合物，这是TGF β I型受体的特征。 通过针对克隆受体的抗体对交联复合物进行免疫沉淀，揭示了70 kd复合物以及94 kd TGF β II型受体复合物。免疫沉淀的新型丝氨酸/苏氨酸激酶受体具有TGF β I型受体的生化特性，并在不同的细胞类型中观察到。将克隆的cDNA转染到TGF β I型受体缺陷细胞中恢复了TGF β诱导的纤溶酶原激活物抑制剂1的产生。这些结果表明，TGF β的信号转导涉及两种不同丝氨酸/苏氨酸激酶受体的异聚复合物的形成。
A cDNA clone encoding a 53 kd serine/threonine kinase receptor with an overall structure similar to that of the type II receptor for transforming growth factor beta (TGFbeta) was obtained. I-125-TGFbeta1 bound to porcine endothelial cells transfected with the cDNA and formed a cross-linked complex of 70 kd, characteristic of a TGFbeta type I receptor. Immunoprecipitation of the cross-linked complexes by antibodies against the cloned receptor revealed the 70 kd complex as well as a 94 kd TGFbeta type II receptor complex. The immunoprecipitated novel serine/threonine kinase receptor had biochemical properties of the TGFbeta type I receptor and was observed in different cell types. Transfection of the cloned cDNA into TGFbeta type I receptor-deficient cells restored TGFbeta-induced plasminogen activator inhibitor 1 production. These results suggest that signal transduction by TGFbeta involves the formation of a heteromeric complex of two different serine/threonine kinase receptors.