MOLECULAR CHARACTERIZATION OF A PROTEIN-TYROSINE-PHOSPHATASE ENRICHED IN STRIATUM
MOLECULAR CHARACTERIZATION OF A PROTEIN-TYROSINE-PHOSPHATASE ENRICHED IN STRIATUM
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DOI：
10.1073/pnas.88.16.7242
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发表时间：
1991-08-01
影响因子：
11.1
通讯作者：
LERNER, M
中科院分区：
文献类型：
--
作者：
LOMBROSO, PJ;MURDOCH, G;LERNER, M
编码神经特异性推定蛋白酪氨酸磷酸酶（蛋白酪氨酸磷酸磷酸水解酶，EC 3.1.3.48）的 cDNA 克隆已从大鼠纹状体 cDNA 文库中分离出来。推导的氨基酸序列预测该蛋白质几乎由 369 个氨基酸组成，与蛋白质酪氨酸磷酸酶家族的其他成员具有很强的同源性。体外翻译产生表观分子质量为 46 kDa 的蛋白质。蛋白质 N 末端的肉豆蔻酰化氨基酸共有序列表明了一种潜在的细胞质膜附着机制。对大鼠大脑各个区域的 RNA 分析揭示了 3 千碱基 (kb) 和 4.4 kb 的 mRNA。相对于其他大脑区域，3-kb mRNA 在纹状体中高度富集，被称为“纹状体富集磷酸酶”(STEP)。相比之下，4.4 kb 的信息在大脑皮层中最丰富，而在纹状体中很少。这两条信息似乎是单个基因的 RNA 转录本的交替处理。
A cDNA clone encoding a neural-specific putative protein-tyrosine-phosphatase (protein-tyrosine-phosphate phosphohydrolase, EC 3.1.3.48) has been isolated from a rat striatal cDNA library. The deduced amino acid sequence predicts a protein of almost-equal-to 369 amino acids with a strong homology to other members of the family of protein-tyrosine-phosphatases. In vitro translation produces a protein with an apparent molecular mass of 46 kDa. A potential attachment mechanism to the cytoplasmic membrane is suggested by a myristoylation amino acid-consensus sequence at the N terminus of the protein. RNA analyses of various regions of rat brain reveal a 3-kilobase (kb) and a 4.4-kb mRNA. The 3-kb mRNA is highly enriched within the striatum relative to other brain areas and has been termed a "striatum enriched phosphatase" (STEP). In contrast, the 4.4-kb message is most abundant in the cerebral cortex and rare in the striatum. These two messages appear to be alternatively processed RNA transcripts of a single gene.