Cellular composition and steroidogenic capacity of the ovary of Macrotus californicus (Chiroptera: Phyllostomatidae) during and after delayed embryonic development.
Cellular composition and steroidogenic capacity of the ovary of Macrotus californicus (Chiroptera: Phyllostomatidae) during and after delayed embryonic development.
复制标题
延迟胚胎发育期间和之后，Macrotus californicus（翼手目：Phyllostomatidae）卵巢的细胞组成和类固醇生成能力。
DOI：
10.1007/bf00318638
复制
发表时间：
1990
影响因子：
3.6
通讯作者：
Krutzsch,PH
中科院分区：
文献类型：
--
作者：
Crichton,EG;Hoyer,PB;Krutzsch,PH
在叶鼻蝠，加利福尼亚巨狐蝠，在3.5个月的正常胚胎发生期（3月至6月）之前，有4.5个月的延迟早期胚胎发生期（10月至3月）。这种延长的妊娠期提供了一个独特的机会，将卵巢变化与植入后的事件联系起来。本研究对妊娠期黄体细胞发育和卵泡生物学进行了研究。植入后循环孕酮(P)水平在过渡到正常发育之前没有变化，并在妊娠活动开始时达到最大值。黄体细胞直径在此期间增加。分娩前血清P水平下降，3β-羟基-5-类固醇脱氢酶-5,4-异构酶（3β-HSD）染色阳性的细胞数量和直径减少，组织切片酶染色强度降低。发育延迟期间存在亚细胞甾体生成细胞器，但恢复正常发育后平滑内质网（SER）明显增加，此时黄体细胞对17β-HSD染色反应阳性。分娩前，脂滴积累和SER减少提示类固醇分泌减少。大的多层卵泡在整个妊娠期均呈3β-HSD阳性，除发育晚期外，17β-HSD阳性。因此，胚胎发生的延迟可能是由于黄体发育不充分或多层卵泡的类固醇生成活性所致。
In the leaf-nosed bat,Macrotus californicus, a 4,5-month period of delayed early embryogenesis (October–March) precedes a 3.5-month period of normal embryogenesis (March–June). This prolonged gestation provides a unique opportunity to correlate ovarian changes with the events following implantation. The present study investigated luteal cell development and follicular biology during gestation. Circulating progesterone (P) levels following implantation were unchanged before transition to normal development, and were maximal at the start of active gestation. Luteal cell diameters increased during this period. Serum P levels declined prior to parturition, when cells staining positive for 3β-hydroxy-5-steroid dehydrogenase-5,4-isomerase (3β-HSD) activity were reduced in number and diameter, and enzyme staining was less intense in tissue slices. Subcellular steroidogenic organelles were present during delayed development, but smooth endoplasmic reticulum (SER) was markedly increased after the resumption of normal development at which time also luteal cells reacted positively to staining for 17β-HSD. Before parturition, lipid droplet accumulation and reduced SER suggested a reduction in steroid secretion. Large multilaminar follicles stained positive for 3β-HSD activity throughout gestation and for 17β-HSD except in late delayed development. Thus, the delay in embryogenesis may be due to an inadequately developed corpus luteum or to the steroidogenic activity of the multilaminar follicles.