Human papillomavirus genotyping and p16INK4a expression in cervical intraepithelial neoplasia of adolescents
Human papillomavirus genotyping and p16INK4a expression in cervical intraepithelial neoplasia of adolescents
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DOI：
10.1038/modpathol.3800290
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发表时间：
2005-02-01
期刊：
影响因子：
7.5
通讯作者：
Hughson, MD
中科院分区：
文献类型：
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作者：
Hu, LL;Guo, M;Hughson, MD
人乳头瘤病毒（HPV）感染是宫颈癌的一种常见类型，也是宫颈癌的一种常见类型。细胞周期蛋白依赖性激酶抑制剂p16（INK 4a）在宫颈上皮内瘤变（CIN）中过表达，可能是由于持续和整合的HPV感染。本研究调查了青少年宫颈活检中p16（INK 4a）表达、CIN分级和高危型HPV感染。对活检组织进行p16（INK 4a）化学染色。通过扩增从宫颈活检中提取的DNA来确定是否存在广谱、低风险或高风险HPV。活检结果：宫颈炎15例，CIN 1级48例，CIN 2级46例，CIN 3级52例。p16（INK 4a）染色的分布分级为斑片状、弥漫基底和弥漫全层。Pearson卡方检验分析p16（INK 4a）染色、HPV感染和CIN之间的关系。宫颈炎活检HPV和p16（INK 4a）表达阴性。高危型HPV 16、18和31型从CIN 1的18%增加到CIN 2/3的66%（P
Adolescents have high rates of human papillomavirus (HPV) infection, and persistent high-risk HPV infection can lead to the development of cervical cancer. The cyclin-dependent kinase inhibitor, p16(INK4a) is overexpressed in cervical intraepithelial neoplasia (CIN), probably due to a persistent and integrated HPV infection. This study investigated p16(INK4a) expression, grades of CIN, and high-risk HPV infection in adolescent cervical biopsies. Biopsies were immunohistochemically stained for p16(INK4a). The presence of wide-spectrum, low-risk, or high-risk HPV was determined by amplifying DNA extracted from the cervical biopsies. Biopsies were classified as cervicitis, 15 cases; CIN 1, 48 cases; CIN 2, 46 cases, and CIN 3, 52 cases. The distribution of p16(INK4a) staining was graded as patchy, diffuse basal, and diffuse full thickness. Pearson's chi(2) tests analyzed the relationships between p16(INK4a) staining, HPV infection, and CIN. Biopsies of cervicitis were negative for HPV and for p16(INK4a) expression. High-risk HPV 16, 18, and 31 increased from 18% in CIN 1 to 66% in CIN 2/3 (P