Sustained Wound Healing Activity of Curcumin Loaded Oleic Acid Based Polymeric Bandage in a Rat Model
Sustained Wound Healing Activity of Curcumin Loaded Oleic Acid Based Polymeric Bandage in a Rat Model
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DOI：
10.1021/mp300075u
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发表时间：
2012-10-01
影响因子：
4.9
通讯作者：
Sahoo, Sanjeeb K.
中科院分区：
文献类型：
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作者：
Mohanty, Chandana;Das, Manasi;Sahoo, Sanjeeb K.
伤口愈合是一个复杂的多阶段过程，包括炎症、细胞增殖、基质沉积和重塑阶段。它通常与氧化应激和随之而来的长期炎症有关，导致伤口愈合受损。据报道，姜黄素可以改善不同动物模型的伤口愈合。为了提高姜黄素在伤口愈合中的作用，研制了姜黄素油酸基聚合物（COP）绷带。在大鼠模型中比较空白绷带和对照组（棉纱布处理）的体内创面愈合能力。生化参数和组织学分析显示，由于其有效的自由基清除特性，COP绷带处理组伤口减少和细胞增殖增强。伤口愈合的相对加速是由于成纤维细胞的早期实施及其分化（α -平滑肌肌动蛋白水平升高）。Western blotting和半定量PCR分析表明，COP绷带能有效抑制自由基，降低抗氧化酶活性。mRNA和蛋白水平的进一步证据表明，我们的系统足以减少伤口愈合过程中由NF κ B途径介导的炎症反应。在这样的背景下，我们预计这种多用途的方法可能会在不久的将来为局部伤口愈合开辟新的领域。
Wound healing is an intricate multistage process that includes inflammation, cell proliferation, matrix deposition and remodeling phases. It is often associated with oxidative stress and consequent prolonged inflammation, resulting in impaired wound healing. Curcumin has been reported to improve wound healing in different animal models. In order to increase the efficacy of curcumin in the healing arena a curcumin loaded oleic acid based polymeric (COP) bandage was formulated. The in vivo wound healing potency was compared with void bandage and control (cotton gauze treatment) in a rat model. Biochemical parameters and histological analysis revealed increased wound reduction and enhanced cell proliferation in COP bandage treated groups due to its efficient free radical scavenging properties. Comparative acceleration in wound healing was due to early implementation of fibroblasts and its differentiation (increased level of alpha-smooth muscle actin). Western blotting and semiquantitative PCR analysis clearly indicate that COP bandage can efficiently quench free radicals leading to reduced antioxidative enzyme activity. Further evidence at mRNA and protein level indicates that our system is potent enough to reduce the inflammatory response mediated by the NF kappa B pathway during wound healing. With this background, we anticipate that such a versatile approach may seed new arena for topical wound healing in the near future.