Radical scavenging activities of Tyr-, Trp-, Cys- and Met-Gly and their protective effects against AAPH-induced oxidative damage in human erythrocytes.
Radical scavenging activities of Tyr-, Trp-, Cys- and Met-Gly and their protective effects against AAPH-induced oxidative damage in human erythrocytes.
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DOI：
10.1016/j.foodchem.2015.11.012
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发表时间：
2016-04
期刊：
影响因子：
8.8
通讯作者：
Lin Zheng;Hongzhu Dong;G. Su;Qiangzhong Zhao;Mouming Zhao
中科院分区：
文献类型：
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作者：
Lin Zheng;Hongzhu Dong;G. Su;Qiangzhong Zhao;Mouming Zhao
本研究评价了酪氨酸、色氨酸、半胱氨酸和蛋氨酸-甘氨酸对自由基的清除作用及其对AAPH诱导的红细胞氧化损伤的保护作用。这种损伤包括溶血、血红蛋白氧化、MDA的形成以及谷胱甘肽（GSH）和过氧化氢酶（CAT）的消耗。结果表明，Tyr-和Trp-Gly能有效地猝灭ABTS和ORAC中的自由基，其TE（Trolox equivalent）值大于1.0 μmol TE/μmol，其次是Cys-和Met-Gly。这些二肽对AAPH诱导的红细胞溶血均有保护作用，且呈剂量依赖性。能显著（p< 0.05）延缓血红蛋白氧化和红细胞GSH耗竭。这些二肽的保护作用顺序为Trp-Gly > Tyr-Gly > Met-Gly > Cys-Gly，这与它们清除过氧化氢自由基的活性一致。提示这些二肽可能通过直接清除自由基而保护红细胞免受AAPH诱导的氧化损伤。
Radical scavenging activities of Tyr-, Trp-, Cys- and Met-Gly and their protective effects against AAPH-induced oxidative damage in erythrocytes were evaluated in this study. This damage includes hemolysis, oxidation of hemoglobin, formation of MDA and the depletion of glutathione (GSH) and catalase (CAT). Results showed that Tyr- and Trp-Gly could quench the radicals effectively in ABTS and ORAC assays with TE (Trolox equivalent) values of more than 1.0 μmol TE/μmol, followed by Cys- and Met-Gly. All these dipeptides could protect erythrocytes against AAPH-induced hemolysis in a dose-dependent manner. They could also significantly (p< 0.05) retard the oxidation of hemoglobin and depletion of GSH in erythrocytes. The protective effects of these dipeptides decreased in the following order: Trp-Gly > Tyr-Gly > Met-Gly > Cys-Gly, which were consistent with their peroxyl radical scavenging activities. It suggested that these dipeptides might protect erythrocytes against AAPH-induced oxidative damage, mainly by acting as the direct radical scavengers.