Fluorescence polarization (FP) assays for the determination of grain mycotoxins (fumonisins, DON vomitoxin and aflatoxins)
Fluorescence polarization (FP) assays for the determination of grain mycotoxins (fumonisins, DON vomitoxin and aflatoxins)
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DOI：
10.2174/138620703106298310
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发表时间：
2003-05-01
影响因子：
1.8
通讯作者：
Jolley, ME
中科院分区：
文献类型：
--
作者：
Nasir, MS;Jolley, ME
荧光偏振分析（FPA）在人类临床、传染病和药物发现领域的成功应用促使我们将其应用扩展到谷物真菌毒素领域。开发了真菌毒素特异性抗体和真菌毒素-荧光团缀合物。游离毒素（用合适的溶剂从谷物中提取）与毒素-荧光团缀合物竞争抗体，并且FP相对于游离毒素的量发生变化。将该变化与使用已知量毒素获得的标准曲线进行比较。使用FP和毒素荧光团共轭物的定量伏马菌素，脱氧雪腐镰刀菌烯醇和黄曲霉毒素的描述。这些测定是现场便携式的，执行简单，快速并且不需要洗涤步骤。
Successful use of fluorescence polarization assays (FPAs) in human clinical, infectious disease, and drug discovery fields has prompted us to extend its use to the grain mycotoxin field. An antibody specific to a mycotoxin and a mycotoxin-fluorophore conjugate are developed. Free toxin (extracted from the grains with a suitable solvent) competes with the toxin-fluorophore conjugate for the antibody and a change in FP relative to the quantity of free toxin occurs. This change is compared to a standard curve obtained by using known quantities of toxin. The use of FP and toxin-fluorophore conjugates for the quantification of fumonisins, deoxynivalenol and aflatoxins is described. These assays are field portable, simple to perform, rapid and require no washing steps.