A 235-bp region from a nutritionally regulated soybean seed-specific gene promoter can confer its sulfur and nitrogen response to a constitutive promoter in aerial tissues of Arabidopsis thaliana
A 235-bp region from a nutritionally regulated soybean seed-specific gene promoter can confer its sulfur and nitrogen response to a constitutive promoter in aerial tissues of Arabidopsis thaliana
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DOI：
10.1016/s0168-9452(02)00064-x
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发表时间：
2002-07-01
期刊：
影响因子：
5.2
通讯作者：
Fujiwara, T
中科院分区：
文献类型：
--
作者：
Awazuhara, M;Kim, H;Fujiwara, T
大豆主要种子贮藏蛋白- - -甘氨酸- -亚基基因的启动子在硫和氮营养下均上调。通过融合花椰菜花叶病毒35S RNA启动子（P35S）增强子下游的β亚基启动子（Pbeta）和β -葡萄糖醛酸酶（GUS）报告基因，在转基因拟南芥非种子组织中研究了这种反应。嵌合启动子在硫缺乏症（AS）下被上调，表明Pbeta能够在非种子组织中指导delta响应。Pbeta的缺失分析表明，在种子中，-307—73 bp区域足以满足delta的响应。在P35S中插入235 bp的区域，使DeltaS在种子和非种子组织中对该组成启动子产生应答。当三个235 bp片段串联插入P35S时，delta的上调程度更为明显。本研究结果表明，硫调控Pbeta在种子和非种子组织中的表达存在一个共同的机制，并且这个235 bp的Pbeta区域足以受到硫和氮营养的调节。(C) 2002爱思唯尔科学爱尔兰有限公司版权所有。
The seed-specific promoter of the beta subunit gene of beta-conglycinin, a major seed storage protein of soybean, is upregulated in response to both sulfur and nitrogen nutrition. This response was studied in non-seed tissues of transgenic Arabidopsis thaliana by fusing the beta subunit promoter (Pbeta) downstream of an enhancer from the cauliflower mosaic virus 35S RNA promoter (P35S) and using the beta-glucuronidase (GUS) reporter gene. The chimeric promoter was upregulated by sulfur deficiency (AS), indicating that the Pbeta is able to direct the DeltaS response in non-seed tissues. Deletion analysis of the Pbeta revealed that, in seeds, the -307--73 bp region is sufficient for the DeltaS response. Insertion of the 235 bp region into the P35S conferred the DeltaS response on this constitutive promoter in both seed and non-seed tissues. The extent of upregulation by DeltaS was more evident when three of the 235 bp fragments were tandemly inserted into the P35S. The findings presented here suggest that a common mechanism exists for sulfur-regulated expression of the Pbeta in both seed and non-seed tissues and that this 235 bp region of the Pbeta is sufficient for regulation by both sulfur and nitrogen nutrition. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved.