A new vacuum-operated stress-providing instrument that applies static or variable duration cyclic tension or compression to cells in vitro.
A new vacuum-operated stress-providing instrument that applies static or variable duration cyclic tension or compression to cells in vitro.
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发表时间：
1985-04
影响因子：
4
通讯作者：
A. Banes;J. Gilbert;D. Taylor;O. Monbureau
中科院分区：
文献类型：
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作者：
A. Banes;J. Gilbert;D. Taylor;O. Monbureau
研制了一种向体外培养的细胞提供循环应力的仪器。该装置使用真空使塑料培养皿变形，通过应变计记录测量，内表面上的细胞产生0.13%的压缩。25 s应激和5 min松弛的方案未诱导与肌动蛋白共迁移的45 × 10（3）Mr蛋白质的合成发生显著变化，而与微管蛋白共迁移的52 × 10（3）Mr蛋白质从对照静止细胞中合成的总蛋白质的12.7 +/- 0.451%下降到应激细胞中的8.53 +/- 0.182%。该装置可广泛应用于监测细胞（如肌肉、肺、肌腱、韧带和骨骼）中响应于应力的生化变化，这些细胞通常受到拉伸或压缩。
An instrument providing cyclic stress to cells cultured in vitro has been developed. The unit uses a vacuum to deform a plastic Petri dish yielding 0.13% compression to cells on the inner surface, measured by strain gauge recordings. A regimen of 25 s stress and 5 min relaxation induced no significant change in synthesis of a 45 X 10(3) Mr protein that comigrates with actin, whereas a 52 X 10(3) Mr protein that comigrated with tubulin decreased from 12.7 +/- 0.451% of the total protein synthesized in control, static cells to 8.53 +/- 0.182% in stressed cells. The unit may have a broad application in monitoring biochemical changes in response to stress in cells such as muscle, lung, tendon, ligament and bone that are normally subjected to tension or compression.