Infection with agents of human granulocytic ehrlichiosis, Lyme disease, and babesiosis in wild white-footed mice (Peromyscus leucopus) in Connecticut
Infection with agents of human granulocytic ehrlichiosis, Lyme disease, and babesiosis in wild white-footed mice (Peromyscus leucopus) in Connecticut
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DOI：
10.1128/jcm.37.9.2887-2892.1999
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发表时间：
1999-09-01
影响因子：
9.4
通讯作者：
Anderson, JF
中科院分区：
文献类型：
--
作者：
Stafford, KC;Massung, RF;Anderson, JF
在1997年和1998年期间，在康涅狄格州南部捕获了白足小鼠，白足鼠，以确定由粒细胞埃立克体属引起的感染的流行率，伯氏疏螺旋体，和巴贝氏菌。在捕获和重新捕获的50只小鼠中，通过间接荧光抗体（IFA）染色方法测定，47只小鼠中有25只（53.2%）和48只小鼠中有23只（47.9%）分别含有BDS或NCH-1埃立克体属菌株的抗体。48只小鼠中的大多数（83.3%）也含有B抗体。通过酶联免疫吸附测定法测定。此外，20/26（76.9%）含有B抗体。用免疫荧光染色法对Microti进行染色。在使用16S rRNA基因的巢式PCR检测中，在47只小鼠中的17只（36.2%）中检测到人粒细胞埃立克体病（HGE）因子的DNA，但在每次捕获时，这17只小鼠中只有4只（23.5%）为PCR阳性。在23只PCR阳性小鼠中的17只（73.9%）中检测到对粒细胞埃立克体属微生物的抗体阳性反应。从9个阳性血液样本的PCR产物的序列是相同的HGE剂。埃立克体属从1998年4月捕获的五只小鼠中的三只中培养，包括1997年4月PCR阳性的一只。此外，附着在两只PCR阳性小鼠身上的14个肩突硬蜱幼虫池中，有2个含有HGE因子的DNA。高百分比的白足小鼠被HGE因子感染或自然感染，在一些个体小鼠中具有低水平的持续感染或频繁的再感染。然而，在几个月的野外采集中，在来自个体小鼠的重复血液和血清样品中存在DNA和抗体的情况下注意到的变化表明，粒细胞埃立克体的感染在大多数野生白足毕赤酵母中是短暂的。
White-footed mice, Peromyscus leucopus, were captured in southern Connecticut during 1997 and 1998 to determine the prevalence of infections caused by granulocytic Ehrlichia sp., Borrelia burgdorferi, and Babesia microti. Of the 50 mice captured and recaptured, 25 of 47 (53.2%) and 23 of 48 (47.9%) contained antibodies to the BDS or NCH-1 Ehrlichia strains, respectively, as determined by indirect fluorescent antibody (IFA) staining methods. The majority (83.3%) of 48 mice also contained antibodies to B. burgdorferi, as determined by enzyme-linked immunosorbent assay. Moreover, 20 of 26 (76.9%) contained antibodies to B. microti by IFA staining methods. In nested PCR tests using the 16S rRNA gene, the DNA of the human granulocytic ehrlichiosis (HGE) agent was detected in 17 of 47 mice (36.2%), but only 4 (23.5%) of these 17 mice were PCR positive at each capture. Antibody-positive reactions to granulocytic Ehrlichia sp. organisms were detected in 17 of 23 (73.9%) of the PCR-positive mice. The sequences from PCR products from nine positive blood samples were identical to the HGE agent. Ehrlichia spp. were cultured from three of five mice captured in April 1998, including one that was PCR positive in April 1997. In addition, 2 of 14 larval Ixodes scapularis pools, which were attached to two PCR-positive mice, contained DNA of the HGE agent. A high percentage of white-footed mice are infected or have been infected naturally by the HGE agent with low-level persistent infection or frequent reinfection in some individual mice. However, the changes noted in the presence of DNA and antibodies in repeated blood and serum samples from individual mice over several months of field collection suggests that infection with granulocytic Ehrlichia is transient in most wild P. leucopus.