Differentiated population structure of a genetically depauperate mangrove species Ceriops tagal revealed by both Sanger and deep sequencing
Differentiated population structure of a genetically depauperate mangrove species Ceriops tagal revealed by both Sanger and deep sequencing
复制标题
桑格和深度测序揭示了基因退化红树林物种 Ceriops tagal 的分化种群结构
DOI：
10.1016/j.aquabot.2012.04.001
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发表时间：
2012-08-01
期刊：
影响因子：
1.8
通讯作者：
Shi, Suhua
中科院分区：
文献类型：
--
作者：
Huang, Yelin;Zhu, Chunchao;Shi, Suhua
Ceriops tagal is a long-lived, woody perennial mangrove species, which is expected to maintain high levels of genetic diversity. We used two kinds of molecular markers, including maternally inherited chloroplast intergenic spacers (total similar to 2550 bp) and bi-parentally inherited nuclear genes (total similar to 69,308 bp) to examine the genetic variation of C. tagal across East India Ocean (EIO), West Pacific Ocean (WPO) and North Australia (NA), representing the largest range ever investigated. For nuclear genes survey, we adopted deep sequencing technologies to sequence 90 nuclear loci from pooled DNA samples of 100 individuals from each of three populations of C. tagal. Our data showed an extremely low level of polymorphism both at the chloroplast regions (nucleotide diversity: pi = 3.2 x 10(-4)) and nuclear loci (pi = 3.0 x 10(-4)) across the species range investigated. This species appeared to have passed through severe and prolonged demographic bottlenecks in the Pleistocene glaciation. However, a highly differentiated population structure among geographical regions was revealed by both molecular markers. Chlorotype analysis identified three major geographical groups in correspond to the EIO, WPO and NA. The high genetic identities within any local populations of C. tagal and shallow distinct genetic differences between geographical groups suggested recent historical isolation among the populations. Genetic information obtained from this study should help provide a framework for the development of conservation program for this species. (C) 2012 Elsevier B.V. All rights reserved.
