The Drosophila gene CG9918 codes for a pyrokinin-1 receptor
The Drosophila gene CG9918 codes for a pyrokinin-1 receptor
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DOI：
10.1016/j.bbrc.2005.07.038
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发表时间：
2005-09-16
影响因子：
3.1
通讯作者：
Grimmelikhuijzen, CJP
中科院分区：
文献类型：
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作者：
Cazzamali, G;Torp, M;Grimmelikhuijzen, CJP
果蝇基因组计划的数据库包含一个基因CG 9918，注释为G蛋白偶联受体的编码。我们克隆了该基因的cDNA，并在中国仓鼠卵巢细胞中进行了功能性表达。我们测试了大约25种果蝇和其他昆虫神经肽的文库，以及表达受体上的7种昆虫生物胺，发现它被低浓度的果蝇神经肽焦激肽-1（TGPSASSGLWFGPRLAamide; EC 50，5 × 10（-8）M）激活。该受体也被其他果蝇神经肽激活，终止于序列PRLamide（Hug-gamma，蜕皮触发激素-1，pyrokinin-2），但在这些情况下，需要约6至8倍的浓度。该受体不被果蝇神经肽激活，所述神经肽含有C-末端PRIamide序列（例如促吐激素-2）或PRVamide（例如capa-1和-2），或其他神经肽和生物胺与焦激肽无关。本文首次证实了CG 9918是果蝇Pyrokinin-1受体基因。(c)2005年爱思唯尔公司All rights reserved.
The database from the Drosophila Genome Project contains a gene, CG9918, annotated to code for a G protein-coupled receptor. We cloned the cDNA of this gene and functionally expressed it in Chinese hamster ovary cells. We tested a library of about 25 Drosophila and other insect neuropeptides, and seven insect biogenic amines on the expressed receptor and found that it was activated by low concentrations of the Drosophila neuropeptide, pyrokinin-1 (TGPSASSGLWFGPRLamide; EC50, 5 x 10(-8) M). The receptor was also activated by other Drosophila neuropeptides, terminating with the sequence PRLamide (Hug-gamma, ecdysis-triggering-hormone-1, pyrokinin-2), but in these cases about six to eight times higher concentrations were needed. The receptor was not activated by Drosophila neuropeptides, containing a C-terminal PRIamide sequence (such as eedysis-triggering-hormone-2), or PRVamide (such as capa-1 and -2), or other neuropeptides and biogenic amines not related to the pyrokinins. This paper is the first conclusive report that CG9918 is a Drosophila pyrokinin-1 receptor gene. (c) 2005 Elsevier Inc. All rights reserved.