Expression of cholesteryl glucoside by heat shock in human fibroblasts
Expression of cholesteryl glucoside by heat shock in human fibroblasts
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热激后人成纤维细胞中胆固醇葡萄糖苷的表达
DOI：
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发表时间：
2000
期刊：
影响因子：
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通讯作者：
K. Murakami‐Murofushi
中科院分区：
文献类型：
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作者：
Shohko Kunimoto;Tetsuyuki Kobayashi;Susumu Kobayashi;K. Murakami‐Murofushi
摘要我们研究了热诱导的人培养细胞TIG-3成纤维细胞糖脂的变化，以显示热休克后甾醇葡萄糖苷的表达。在暴露于高温（42°C）15和30分钟的TIG-3细胞的脂质提取物中，在薄层色谱板上检测到糖脂条带，而在没有热休克的情况下几乎检测不到。胆固醇和葡萄糖几乎都是通过气液色谱法作为脂质的降解产物检测到的。脂质分子的结构通过电喷雾质谱法被阐明为胆固醇葡糖苷。这是第一份报告显示哺乳动物细胞中存在甾醇葡萄糖苷，这种物质被认为在哺乳动物细胞的热休克反应中具有重要作用。
Abstract We investigated the heat-induced alteration of glycolipids in human cultured cells, TIG-3 fibroblasts, to show the expression of steryl glucoside by heat shock. A glycolipid band was detected on a thin-layer chromatography plate in lipid extracts from TIG-3 cells exposed to high temperature (42°C) for 15 and 30 minutes, while it was hardly detectable without heat shock. Both cholesterol and glucose were almost exclusively detected by gas liquid chromatography as degradation products of the lipid. The structure of the lipid molecule was elucidated by electrospray mass spectrometry to be a cholesteryl glucoside. This is the first report to show the occurrence of a steryl glucoside in mammalian cells, and this substance is considered to have a significant role in heat shock responses in mammalian cells.