SARS-CoV-2 RNA detection in the air and on surfaces in the COVID-19 ward of a hospital in Milan, Italy
SARS-CoV-2 RNA detection in the air and on surfaces in the COVID-19 ward of a hospital in Milan, Italy
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DOI：
10.1016/j.scitotenv.2020.140540
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发表时间：
2020-11-10
影响因子：
9.8
通讯作者：
Balzaretti, Claudia M.
中科院分区：
文献类型：
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作者：
Razzini, Katia;Castrica, Marta;Balzaretti, Claudia M.
COVID-19疫情在全球范围内迅速发展，发现卫生系统，科学家和社会没有准备好面对一种鲜为人知，快速传播和极其致命的病毒。意大利是受疫情影响最严重的国家之一，导致医疗机构承受沉重负担和严格限制措施。尽管努力澄清病毒传播，特别是在室内情况下，SARS-CoV-2传播的几个方面仍然是初步的。这项研究评估了意大利米兰一家医院COVID-19隔离病房中SARS-CoV-2 RNA对空气和表面的污染。在病房的五个不同区域，包括污染区（COVID-19患者区）、半污染区（更衣室）和院长区，共采集了42份空气和表面样本。采用真实的实时逆转录聚合酶链反应（RT-PCR）检测SARS-CoV-2 RNA。总的来说，24.3%的拭子样本为阳性，但无一在清洁区采集。因此，污染区（35.0%）和半污染区（50.0%）的阳性率高于清洁区（0.0%; P
The COVID-19 outbreak has rapidly progressed worldwide finding the health system, scientists and society unprepared to face a little-known, fast spreading, and extremely deadly virus. Italy is one of the countries hardest hit by the pandemic, resulting in healthcare facilities bearing heavy burdens and severe restrictive measures. Despite efforts to clarify the virus transmission, especially in indoor scenarios, several aspects of SARS-CoV-2 spread are still rudimentary. This study evaluated the contamination of the air and surfaces by SARS-CoV-2 RNA in the COVID-19 isolation ward of a hospital in Milan, Italy. A total of 42 air and surface samples were collected inside five different zones of the ward including contaminated (COVID-19 patients' area), semi-contaminated (undressing room), and dean areas. SARS-CoV-2 RNA detection was performed using real time reverse transcription polymerase chain reaction. Overall, 24.3% of swab samples were positive, but none of these were collected in the clean area Thus, the positivity rate was higher in contaminated (35.0%) and semi-contaminated (50.0%) areas than in clean areas (0.0%; P