SEM observations of Retzius lines and prism cross-striations in human dental enamel after different acid etching regimes
SEM observations of Retzius lines and prism cross-striations in human dental enamel after different acid etching regimes
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DOI：
10.1016/s0003-9969(03)00195-x
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发表时间：
2004-01-01
影响因子：
3
通讯作者：
Risnes, S
中科院分区：
文献类型：
--
作者：
Li, CF;Risnes, S
目标和设计：切片和研磨的牙釉质表面需要用酸蚀刻，以使牙釉质结构暴露在SEM中进行研究。在本研究中，Retzius尖齿和棱镜横纹观察后，用不同浓度的硝酸（0.1，1，2.5和5%）和不同的时间段（15，30，45，90和180秒）蚀刻后的研磨标本。其目的是提高我们对这些结构的解释，并找到最佳的蚀刻制度。结果与结论：釉质表层的Retzius线比深层的Retzius齿更容易暴露。前者表现出不连续的缺陷，便于他们的识别，而后者没有这样的缺陷。暴露的深Retzius齿很少，在1%酸中蚀刻30 s后可以首先确定。深层雷兹尤斯线的可见性的结构和组成基础可能是微妙的，难以识别。切片/研磨程序产生的碎屑可能会干扰其识别，但一般而言，研磨程序对釉质结构的解释不太重要。棱镜交叉条纹更容易暴露于三个较强的酸浓度比最弱的。它们呈现为交替的紧密和暗带，在暗带中具有较低的晶体浓度。用1%硝酸蚀刻30 s似乎是Retzius尖齿和棱镜横纹研究的一个很好的折衷。(C)2003 Elsevier Ltd.保留所有权利。
Objective and design: Sectioned and ground enamel surfaces need to be etched with acid in order for the enamel structure to be exposed for study in the SEM. In the present study Retzius tines and prism cross-striations were observed in variably ground specimens after etching with nitric acid of different concentrations (0.1, 1, 2.5 and 5%) and for different time periods (15, 30, 45, 90 and 180 s). The aims were to improve our interpretation of these structures and to find the best etching regime. Results and conclusions: Retzius lines in the superficial enamel were more readily exposed than Retzius tines in deeper parts of the enamel. The former showed discontinuity defects that facilitated their identification, while the latter lacked such defects. Few deep Retzius tines were exposed and could first be identified with certainty after etching for 30 s in 1% acid. The structural and compositional basis for the visibility of deep Retzius lines may be subtle and difficult to identify. Scratches from the sectioning/grinding procedures may disturb their identification, but in general the grinding procedure was of minor importance for interpretation of enamel structure. Prism cross-striations were more readily exposed with the three stronger acid concentrations than with the weakest. They appeared as alternate tight and dark bands, with a lower crystal concentration in the dark bands. No clear association with the prism varicosities/undulations was found. Etching with 1% nitric acid for 30 s seems to be a good compromise for the study of Retzius tines and prism cross-striations. (C) 2003 Elsevier Ltd. All rights reserved.