Genetic diversity and relationships of sweetpotato and its wild relatives in Ipomoea series Batatas (Convolvulaceae) as revealed by inter-simple sequence repeat (ISSR) and restriction analysis of chloroplast DNA
Genetic diversity and relationships of sweetpotato and its wild relatives in Ipomoea series Batatas (Convolvulaceae) as revealed by inter-simple sequence repeat (ISSR) and restriction analysis of chloroplast DNA
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DOI：
10.1007/s001220051386
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发表时间：
2000-05-01
影响因子：
5.4
通讯作者：
Sun, M
中科院分区：
文献类型：
--
作者：
Huang, JC;Sun, M
利用ISSR标记以及叶绿体DNA四个非编码区的酶切位点变异，对代表甘薯组（series Batatas）十个物种的40份甘薯属（Ipomoea）材料的遗传多样性及亲缘关系进行了研究。用15对引物在这些材料中共产生2071条ISSR片段，平均每个材料扩增出52条带。多数引物包含二核苷酸重复序列。在所研究的40份材料中，ISSR片段具有高度多态性（62.2%）。对叶绿体（cp）DNA的酶切分析揭示出47个有信息的酶切位点和长度突变。对ISSR和cpDNA数据集的系统发育分析在种间水平上通常显示出相似的亲缘关系，但ISSR的高度多态性使得种内材料能更好地区分。然而，ISSR和cpDNA数据集相结合似乎更适合解决种内和种间的亲缘关系。在所研究的物种中，发现三浅裂野牵牛（I. trifida）与栽培甘薯（六倍体的甘薯I. batatas）亲缘关系最近，而多毛野牵牛（I. ramosissima）和荫生野牵牛（I. umbraticola）在该组中与甘薯亲缘关系最远。基于ISSR相似性指数，迄今为止被认为是甘薯祖先之一的三裂叶野牵牛（Ipomoea triloba）与甘薯的亲缘关系较远。
Genetic diversity and relationships of 40 accessions of Ipomoea, representing ten species of series Batatas, were examined using ISSR markers and restriction-site variation in four non-coding regions of chloroplast DNA. A total of 2071 ISSR fragments were generated with 15 primers in these accessions and, on average, 52 bands per accession were amplified. Most of the primers contained dinucleotide repeats. The ISSR fragments were highly polymorphic (62.2%) among the 40 accessions studied. Restriction analysis of chloroplast (cp) DNA revealed 47 informative restriction-site and length mutations. Phylogenetic analyses of ISSR and cpDNA datasets generally revealed similar relationships at the interspecific level, but the high polymorphism of ISSRs resulted in a better separation of intraspecific accessions. However, the combined ISSR and cpDNA dataset appeared to be appropriate in resolving both intra- and interspecific relationships. Of the species examined, I. trifida was found to be the most closely related to cultivated sweetpotato, the hexaploid I. batatas, while I. ramosissima and I. umbraticola were the most distantly related to I. batatas within the series. Ipomoea triloba, hitherto considered to be one of the ancestors of sweetpotato, was only distantly related to sweetpotato based on ISSR similarity index.